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Biocell ; 35(1): 35-36, Apr. 2011.
Article in English | LILACS | ID: lil-595003

ABSTRACT

E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.


Subject(s)
Humans , Animals , Dogs , DNA, Bacterial/analysis , Ehrlichia canis/genetics , Ehrlichia canis/isolation & purification , Dog Diseases/microbiology , Cell Separation/instrumentation , Cell Separation/methods , Cell Line , Fluorescent Dyes/metabolism , Organic Chemicals/metabolism , Ehrlichiosis/microbiology , Glass , Polymerase Chain Reaction/methods
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