ABSTRACT
Objectives: This study aimed at assessing the effect of Gum Arabic as Prebiotics and lactobacillus casei shirota (LcS) as probiotic on oxidative stress and renal function in adenine–induced chronic renal failure in rats. Methodology: 70 male albino rats were divided into 7 groups and treated for 8 weeks as follows group 1: control basal diet group (BD), group 2: adenine in feed (0.75%, w/w), group 3: gum Arabic (GA) in drinking water (15%, w/v), group 4: lactobacillus casei shirota (LcS) 1 x 109 colony-forming units (CFU) supplement, group 5 adenine + GA, as before, group 6 adenine +(LcS) as before and group 7: adenine + GA+ (LcS) as before. Urine, blood and kidneys were collected from the rats at the end of the treatment for analysis of conventional renal function tests serum creatinine, urea, uric acid, sodium, potassium concentration). In addition, the oxidative stress markers serum and kidney glutathione and superoxide dismutase, serum catalase and malondialdhyde (MDA) were measured. Results: By the end of the 8 weeks of treatment, Adenine significantly (p <0.05) increased the concentrations of serum creatinine, urea, uric acid, sodium, potassium and serum MDA. In addition, the oxidative stress markers serum and kidney glutathione and superoxide dismutase, serum catalase was, significantly decreased. Treatment with (GA) and (LcS) significantly ameliorated these actions. The mechanism of the reported salutary effect of GA in adenine-induced CRF is associated with mitigation of the adenine- induced inflammation and generation of free radicals. Conclusion: The results suggest that that oral administration of gum Arabic and lactobacillus casei shirota could conceivably alleviate adverse effects of adenine induced renal toxicity (CRF).