[Effect of referral of cancer patients to medical centers outside Golestan province on the coverage and quality of population-based cancer registry data]

Background and Objective: Considering the importance of coverage and quality of data in Golestan population-based Cancer Registry [GPCR], northern Iran. This study was conducted to determine the effect of the referral of cancer patients to medical centers outside Golestan province on the coverage of the population-based cancer registry program

Methods: This descriptive-analytic study was performed on all cancer patients registered in the diagnostic centers of Golestan province, northern Iran during 2008-10. Data of patients referred to the centers of the province from the bank of the Golestan province cancer registry program and data of patients referred to the centers outside the province were received from the Ministry of Health Department of Cancer. Data were analyzed using the record linkage method

Results: A total of 6083 new cases of cancer were registered in Golestan province. According to the cancer data, 678 [11%] of them were referred to outside of Golestan province centers. 343 of these 678 patients [50.6%] were found as duplicate records between the two datasets. The most frequent cases were gastric cancers [70.4%], breast [63.5%] and esophagus [62.5%]

Conclusion: This study suggested that referrals of cancer patients to centers outside the Golestan province may result in an underestimation of about 5% in GPCR. This level of underestimation seems to have no considerable effects on completeness and quality of data in the GPCR

Hesa-a effects on cell cycle signaling in esophageal carcinoma cell line

Background: Hesa-A is a natural compound with anticancer properties. The exact mechanism of its action in esophageal cancer is not clear, yet. The aim of this study was to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell lines, KYSE-30, and cell cycle genes expression

Methods: In this study, we tested cell toxicity with MTT [3-[4,5-Dirnethylthiazol-2-yl]-2,5-Diphenyltetrazolium Bromide] assay and flow cytometry to evaluate the cell cycle arrest

Real time polymerase chain reaction was used to assess the expression of P53, PI6, P21, cyclin Dl, and cyclin Bl genes

Results: Our results showed that Hesa-A is effective in the expression of cell cycling check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell cycle. The levels of P53 [>13 times], P21 [>21 times], PI6, cyclin Bl, and cyclin Dl genes were increased 48 hours after Hesa-A treatment

Conclusion: P21 and P16 expression were the potential mechanisms for G2 arrest of KYSE-30 esophageal cancer cell line by Hesa-A