Study of alkaline phosphatase from human hydatidiform mole

Study was performed on purification of alkaline phosphatase from Hydatiform mole. The sample of mole Hydatidiform subjects from Taleghani Hospital, Tehran, Iran was studied. An Alkaline phosphatase from Human Hydatidiform mole was purified by a protocol involving solubilization using precipitation by butanol, acetone, Ammonium sulphate, Sephadex G[200], Ion exchange chromatography and preparative electrophoresis. The enzyme was purified 800-fold to apparent homogeneity. This enzyme has 5.2% carbohyrate content. The optimum Temperature and pH were 40 centigrade degree and 10.4, respectively. Human Hydatidiform mole alkaline phosphatase is a novel alkaline phosphatase