ABSTRACT
The study aimed to investigate the antihyperglycemic and antilipidperoxidative effects of ethanol extract of the dry matter from culture broth of Coriolus versicolor (ECBC) in streptozotocin-induced diabetes mice. Blood glucose level, insulin level, total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol(LDL-C), high density lipoprotein -cholesterol (HDL-C) in serum and reduced glutathione level (GSH), lipid peroxidation, glycogen, antioxidant enzymes in liver were evaluated. Moreover, histopathological observation was conducted. Streptozotocin treatment (150 mg/kg body weight) induced the decrease of GSH level, antioxidant enzymes activities, glycogen content in liver, HDL-C content and insulin level in serum, accompanied by the elevation of the lipid peroxidation in liver, serum blood glucose level, contents of TC, TG and LDL-C. Treatment with ECBC restored the changes in the above parameters up to the basal level. The protective effects were further supported by the attenuation of the degree of pancreas damage in ECBC treated mice.
ABSTRACT
The objective of this work was to study the effect of caffeine and tannins on Pleurotus sp. cultivation and to evaluate the feasibility of using coffee husks as substrate for mushroom cultivation. Eight strains of P. ostreatus and two strains of P. sajor-caju were screened on a medium prepared from agar extract of coffee husk. Based on best mycelial growth and biomass production, the strain P. ostreatus LPB 09 was selected for detailed studies. With the increase of caffeine concentration, the mycelial growth and the biomass production decreased, and no growth was observed when concentration of caffeine was 2500 mg/L. Furthermore, Pleurotus did not degrade the caffeine, but absorbed it. Tannin under 100 mg/L in the medium stimulated the growth of mycelia, but above 500 mg/L it had a negative effect. When the concentration reached 1000 mg/L, the fungus still survived and showed a certain tolerance to it. No tannic acid was found in the mycelia, but its concentration decreased in the medium. This fact confirmed that Pleurotus had the capacity of degrading tannic acid. Fructification occurred after 20 days of inoculation and the biological efficiency reached about 97 percent after 60 days. Caffeine content in the husk after cultivation was reduced to 60.7 percent and tannins to 79.2 percent. The results indicated the feasibility of using coffee husk without any pretreatment for the cultivation of Pleurotus.
O objetivo do presente trabalho foi estudar o efeito da cafeína e taninos sobre o desenvolvimento do fungo Pleurotus sp. e avaliar a possibilidade do uso da casca de café como substrato no seu cultivo. Oito cepas de P. ostreatus e duas cepas de P. sajor-caju foram selecionadas em um meio à base de extrato aquoso de casca de café e Agar. A cepa P. ostreatus LPB 09 foi escolhida para estudos posteriores com base na velocidade de crescimento do micélio e produção de biomassa. O estudo do desenvolvimento do cogumelo em meio com diferentes concentrações de cafeína mostrou que o aumento da concentração da mesma influencia negativamente crescimento. A partir de uma concentração de cafeína de 2500 mg/L não foi observado crescimento. Observou-se ainda que o Pleurotus não degradou a cafeína, mas a absorveu. Os taninos em concentração abaixo de 100 mg/L no meio estimularam o crescimento do micélio, no entanto acima de 500 mg/L mostraram efeito negativo. Quando a concentração de taninos atingiu 1000 mg/L, o cogumelo ainda sobreviveu e mostrou uma certa tolerância. O ácido tânico não foi encontrado no micélio, o que confirma que o Pleurotus possui a capacidade de degradá-lo. A frutificação ocorreu após 20 dias de inoculação e a eficiência biológica atingiu 97 por cento em 60 dias. O conteúdo de cafeína da casca de café foi reduzido para 60.7 por cento e o de taninos para 79.2 por cento. Os resultados mostram boas perspectivas para o cultivo de Pleurotus utilizando casca de café como substrato sem a necessidade de qualquer pré-tratamento.