ABSTRACT
OBJECTIVE: To study the chemical constituents in the fruits of Gardenia jasminoides var. radicans Makino. METHODS: Percolation with alcohol and liquid-liquid extraction methods were used to extract the constituents. And silica gel, reverse-phase octadecylsilyl(ODS), and sephadex LH-20 chromatographic methods were applied to isolate and purify compounds. MS, NMR spectroscopic methods were used to elucidate their structures. RESULTS: Ten compounds were isolated from the ethyl acetate fraction of the ethanolic extract of fruits from Gardenia jasminoides var. radicans Makino, and their structures were identified as seven iridoids: geniposide (1), 6α-hydrogeniposide (2), 6β-hydrogeniposide (3), 6α-methoxygeniposide (4), 6β-methoxygeniposide (5), 2'-O-(4″-Hydrooxycinnamoyl) mussaenosidic acid(6), 6″-O-trans-p-coumaroylgenipin genitiobioside(7); two quinic acid derivatives: 3, 5-di-O-caffeoyl-4-O-(3-hydroxyl-3-methyl) glutaroylquinic acid(8), methyl 3, 5-di-caffeoyl-4-O-(3-hydroxy-3-methyl)-glutaroylquinate(9); and one flavonoid: quercetin-3-O-β-D-glucopyranoside(10). CONCLUSION: Compounds 3-10 are isolated from Gardenia jasminoides var. radicans Makino for the first time.
ABSTRACT
To investigate the chemical constituents from Commelina communis, fifteen compounds were separated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and semi-preparative HPLC. By analyses of NMR and MS data as well as their physical and chemical properties, the structures of these compounds were identified as chrysoeriol-7-O-beta-D-glucoside( 1), methyl gallate(2), p-coumaric acid(3), protocatechuic acid(4), caffeic acid(5), p-hydroxybenzoic acid(6), 2-phenethyl-beta-D-gly-cosidase(7) , rhaponticin(8) , (7S, 8R) -dihydrodehydrodiconiferyl alcohol-9-O-beta-D-glucoside (9), isovitexin (10) , isofurcatain (11), isorhamnetin-3-O-beta-D-glucoside(12) , quercetin-3-O-alpha-L-rhamnoside (13) , isoquercitrin (14) , and 1, 2-dihydro-6, 8-dime-thoxy-7-1-(3, 5-dimethoxy-4-hydroxyphenyl) -N1, N2-bis-[2-( 4-hydroxyphenyl) ethyl] -2, 3-naphthalene dicarboxamide (15). Compounds 2, 5-9, 11, 13 were obtained from the genus Commelina for the first time.