ABSTRACT
Objective: To study the chemical constituents from the medicinal herb of Caesalpinia decapetala. Methods: Silica gel and Sephadex LH-20 column chromatography techniques were used as pretreatments for ethanol extract of the seeds of C. decapetala, and then semi-preparative HPLC technique was used. Results: Eight cassane diterpenes were isolated from the chloroform extract of C. decapetala. According to NMR, ESI-MS, and CD spectrum data, they were identified as 1α,6α,7β-triacetoxy-14α-methoxy- vouacapen-5α-ol (1), caesalmin F (2), neocaaesalpin MP (3), 1-deacteoxy-1-oxocaesalmin C (4), neocaesalpin AA (5), bonducellpin C (6), bonducellpin E (7), and neocaaesalpin N (8). Conclusion: Compound 1 is a new compound and named as caediterpene A, compound 1-2 are a pair of epimeric diterpenes, and compounds 3-7 are obtained for the first time from the seeds of C. decapetala.
ABSTRACT
Seven cucurbitane-type triterpenoids were isolated from the ethanol extract of the tubers of Hemsleya dolichocarpa, with a combination of various chromatographic approaches, including silica gel, Sephadex LH-20, Semi-HPLC and so on. On the basis of spectroscopic data analysis, they were identified as 3β,11α,26,27-tetrahydroxycucurbita-5,24(E)-diene-3,26-glucosides (1), scandenogenin D (2), jinfushanencin F (3), scandenoside R3 (4), scandenoside R1 (5), scandenogenin A (6), scandenoside R2 (7). Among them, compound 1 is a new triterpenoid, compound 2 showed remarkable activity against human cancer cell line HeLa with IC50 value of 6.78 μmol·L-1.
ABSTRACT
Objective According to the folk application, the chemical constituents of the seeds of Caesalpinia decapetala were studied. Methods Silica gel and Sephadex LH-20 column chromatography techniques were used as pretreatments for ethanol extract of the seeds of C. decapetala, and then semi-preparative HPLC technique was used. Structures were identified by NMR and MS spectra. Results Five cassane-type diterpenes were isolated from the chloroform extract of C. decapetala. According to NMR and MS spectra data, the isolated compounds were identified as caesalmin F1 (1), α-caesalpin (2), caesalmin F (3), caesalmin C (4), and caesalmin E (5). Conclusion Compound 1 is a new compound, and compounds 1, 4 and 5 are isolated for the first time from C. decapetala. Compounds 1, 4 and 5 show moderate anti-tumor activity against HeLa cells with IC50 values of 64.3, 42.9, and 81.2 μmol/L, respectively.
ABSTRACT
AIM:To investigate the effect of high mobility group box-1 protein (HMGB1) on the expression of nuclear factor-κB ( NF-κB) in BV-2 cells stimulated with amyloid β-protein ( Aβ) 25-35 .METHODS:Cultured BV-2 cells in logarithmic growth phase were divided into 4 groups:normal cell group ( without any treatment ) , model group ( treated with Aβ25-35 at 40 μmol/L) , RNA interference ( RNAi) group ( conducted with HMGB1-siRNA followed by Aβ25-35 stimula-tion) and solvent control group (treated with 0.1% DMSO).After treatment with Aβ25-35 for 24 h, the protein levels of HMGB1 and NF-κB in BV-2 cells were determined by Western blot .RESULTS:Aβ25-35 at 40μmol/L was used to stimu-late BV-2 cells.The GFP fluorescence-tagged HMGB1-siRNA (30 nmol/L) was used to transfect BV-2 cells and its trans-fection efficiency was about 80%~90%.The results of Western blot showed that the protein level of HMGB 1 was signifi-cantly decreased after the interference of siRNA fragment (P<0.05).The protein levels of HMGB1 and nucleic NF-κB p65 were dramatically increased in BV-2 cells stimulated with Aβ25-35(P<0.05).After RNA interference with HMGB1, the expression of HMGB1 and nucleic NF-κB p65 were significantly decreased in BV-2 cells stimulated with Aβ25-35 ( P<0. 05).CONCLUSION:RNA interference with HMGB1 reduces the expression of nucleic NF-κB in BV-2 cells stimulated with Aβ25-35 .