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Objective To find out the dietary patterns and explore the relationship between environmental factors (especially dietary patterns) and diabetes mellitus in the adults of Fujian.Methods Multi-stage sampling method were used to survey residents aged ≥18 years by questionnaire,physical examination and laboratory detection in 10 disease surveillance points in Fujian.Factor analysis was used to identify the dietary patterns,while logistic regression model was applied to analyze relationship between dietary patterns and diabetes mellitus,and classification tree model was adopted to identify the influencing factors for diabetes mellitus.Results There were four dietary patterns in the population,including meat,plant,high-quality protein,and fried food and beverages patterns.The result of logistic analysis showed that plant pattern,which has higher factor loading of fresh fruit-vegetables and cereal-tubers,was a protective factor for non-diabetes mellitus.The risk of diabetes mellitus in the population at T2 and T3 levels of factor score were 0.727 (95％CI:0.561-0.943) times and 0.736 (95％ CI:0.573-0.944) times higher,respectively,than those whose factor score was in lowest quartile.Thirteen influencing factors and eleven group at high-risk for diabetes mellitus were identified by classification tree model.The influencing factors were dyslipidemia,age,family history of diabetes,hypertension,physical activity,career,sex,sedentary time,abdominal adiposity,BMI,marital status,sleep time and high-quality protein pattern.Conclusion There is a close association between dietary patterns and diabetes mellitus.It is necessary to promote healthy and reasonable diet,strengthen the monitoring and control of blood lipids,blood pressure and body weight,and have good lifestyle for the prevention and control of diabetes mellitus.
ABSTRACT
Objective To find out the dietary patterns and explore the relationship between environmental factors (especially dietary patterns) and diabetes mellitus in the adults of Fujian.Methods Multi-stage sampling method were used to survey residents aged ≥18 years by questionnaire,physical examination and laboratory detection in 10 disease surveillance points in Fujian.Factor analysis was used to identify the dietary patterns,while logistic regression model was applied to analyze relationship between dietary patterns and diabetes mellitus,and classification tree model was adopted to identify the influencing factors for diabetes mellitus.Results There were four dietary patterns in the population,including meat,plant,high-quality protein,and fried food and beverages patterns.The result of logistic analysis showed that plant pattern,which has higher factor loading of fresh fruit-vegetables and cereal-tubers,was a protective factor for non-diabetes mellitus.The risk of diabetes mellitus in the population at T2 and T3 levels of factor score were 0.727 (95％CI:0.561-0.943) times and 0.736 (95％ CI:0.573-0.944) times higher,respectively,than those whose factor score was in lowest quartile.Thirteen influencing factors and eleven group at high-risk for diabetes mellitus were identified by classification tree model.The influencing factors were dyslipidemia,age,family history of diabetes,hypertension,physical activity,career,sex,sedentary time,abdominal adiposity,BMI,marital status,sleep time and high-quality protein pattern.Conclusion There is a close association between dietary patterns and diabetes mellitus.It is necessary to promote healthy and reasonable diet,strengthen the monitoring and control of blood lipids,blood pressure and body weight,and have good lifestyle for the prevention and control of diabetes mellitus.
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Objective To establish an HPLC method to determine the related substances of metolazone and valsartan in com?pound metolazone tablets. Methods An Agilent Eclipse SB-C18 column (4.6 mm × 250 mm,5 μm) was used with 0.01 mol/L KH2PO4 buffer(pH=3.5)-acetonitrile as the mobile phase with gradient elution at a flow rate of 1.0 ml/min. The column temperature was 30℃ and the detection wavelength was 237 nm. Injection volume was 20 μl. Results Metolazone,valsartan and related sub?stance B of valsartan were separated completely. The calibration curves were linear within the range of 3-30μg/ml for metolazone, 0.1-2.0μg/ml for valsartan and 0.08-2.0μg/ml for related substane B of valsartan. The average recoveries were 102.97%,100.81%and 100.44%,respectively. The repeatability and intermediate precision met with requirements. The test solution was stable within 24 h. Conclusion The method is specific,sensitive,accurate and reliable,thereby can be used for the determination of metolazone and valsartan related substances in compound metolazone tablets.
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Objective To investigate the anti-tumor activity of long-circulation thermosensitive liposom-loaded vincristine (VTSL)in vivo and in vitro. Methods The inhibitory effects of VTSL and vincristine injection(VCR)on sw620 cell and PANC cell were detected by MTT assay. The uptake capacity of HT-1080 was studied by using Cou-6-loaded liposome. Different xenograft nude mice models of HepG-2 and MCF-7 were established. To study anti-tumor effect of VTSL,drugs were given via tail and heat therapeu?tic area for 30 minutes,mice weight and tumor weight were recorded. To study anti-tumor effect of VTSL,drugs were given via tail vein and heat therapeutic areas for 30 min,mice body mass and tumor mass were recorded. Results After VTSL was given 72 h,the activ?ity of sw620 and PANC was less than 5%and 20%,respectively. VTSL showed stronger cytotoxic effect than vincristine. At the same dose,tumor inhibitory rate of VCR and VTSL on HepG-2 and MCF-7 bearing nude mice was 50%,69.7%,47.8%and 76.1%,respec?tively. There were significant differences in tumor weight after treatment. Conclusion VTSL enhances the cytotoxicity by heating. Loading vincristine into TSL increased cytotoxicity,and heating could promote the fusion of liposomes and cell membrane. Under the same dosage,VTSL showed much higher tumor inhibition rate than VCR,and there was a certain dose dependence. The results show that VTSL can be used in treatment of solid tumor and has the potential to expand vincristine clinical application.
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Objective To establish and validate the assay methods of release,content,content uniformity and related sub?stances of desvenlafaxine succinate (DVS) in extended-release tablets. Methods The ultraviolet spectrophotometric method was used to determine the DVS release from DVS extended-release tablets. The content uniformity,content and related substance were deter?mined by high-performance liquid chromatography(HPLC). To validate all the method,we respectively examined specificity,linearity, recovery rate,precision and stability,etc. Results The results showed that the analysis method for release was specific,the calibra?tion curve was linear in the range of 10-200μg/ml,and all the recovery,repeatability and intermediate precision met requirements. The method for detection of content and content uniformity was specific and linear in the range of 5-400μg/ml,the recovery,repeat?ability and intermediate precision met requirements. The method for related substances was specific and sensitive ,the linear and recovery rate met the requirements. All of the solutions were stable during 24 h at room temperature. Conclusion The analysis meth?od for release is simple,sensitive,specific and accurate,the method for content and content uniformity is accurate and reliable,and the method for related substances is specific,sensitive and accurate. These methods are suitable for quality control of DVS extended-release tablets.
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Objective To establish and validate a LC-MS/MS method for quantitative analysis of a new anti-stroke compound TID-101 in rat plasma and to study the pharmacokinetics and bioavailability of TID-101 self-(micro)emulsified drug delivery system (SMEDDS). Methods The plasma samples were treated with methanol for precipitating protein. The chromatographic separation was achieved with a acetonitrile-water mobile phase. Detection of TID-101 and the internal standard (IS) dexamethasone acetate was achieved by electrospray ionization(ESI)source in the negative ion mode at m/z 353.4→323.2 and m/z 433.4→353.4. The method was applied for pharmacokinetics study of TID-101 between SMEDDS in rats. Results The method was linear over TID-101 concen?tration range from 10-95 000 ng/ml with the correlation coefficients(r)of 0.9998. The intra-run and inter-run relative standard devia?tions(RSD)were less than 15%and the average absolute recovery values were 83.4-87.0%. The validated method was applied to a pharmacokinetic study in rats after intravenous administration of TID-101 fat emulsion injection and oral administration of TID-101 suspension and SMEDDS. The bioavailability of TID-101 API and SMEDDS was 2.8% and 14.9%,respectively. Conclusion The analysis method is simple,accurate,and sensitive for assaying the in vivo pharmacokinetic study of TID-101 in rats. SMEDDS could effectively enhance the oral bioavailability of TID-101.
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An on-line solid phase extraction ( SPE ) coupled with HPLC-MS/MS method was developed to determine S-ammuxetine and R-ammuxetine in rat plasma. The sample preparation consisted of the following steps:A protein precipitation extraction used methanol and acetonitrile ( 50:50 , V/V ); an on-line SPE treatment to remove most matrixes in plasma;an enrichment and separation step used a C18 analytical column. S-and R-ammuxetine were determined by tandem mass spectrometry. The SPE column was a Retain PEP Javelin (10 mm × 2. 1 mm × 5 μm), while the chromatographic separation was achieved using a ZORBAX SB-C18 (50 mm × 2. 1 mm × 3. 5 μm) analytical column with an isocratic mobile phase composed of acetonitrile-water-formic acid (40:60:0. 1, V/V/V, 0. 3 mL/min). The selected reaction monitoring mode of the positive ion was performed and the precursor to the product ion transitions of m/z 292 . 1/154 . 0 and m/z 260. 4/116. 2 were used to measure S-ammuxetine, R-ammuxetine and internal standard (propranolol). The method was linear over a concentration range from 0 . 2 to 1000 μg/L with the correlation coefficients of 0 . 9903 and 0 . 9951 . The average intra-day precision values were 1 . 2% -12 . 0% for S-ammuxetine and 0. 4%-11. 2% for R-ammuxetine, respectively. The average recoveries were 94. 2%-101. 6% for S-ammuxetine and 94. 3% -109. 4% for R-ammuxetine. Compared to the literature, the sensitivity of this method increased dramatically. The present method has been successfully applied to the preclinical rat research of ammuxetine isomers following intragastric administration.