ABSTRACT
Objective To investigate the effect of exercise diary on arm exercise compliance ofpatients with peripherally inserted centralcatheter (PICC). Methods Thirty patients with PICC hospitalized in our hospital from June to August in 2017 were selected as the control group, where the patients received guidance on functional exercises through arm exercise videos and routine methods. Another thirty-two patients hospitalized in our hospital from September to November of 2017 were selected as the observation group, where in addition to the same treatment as in the control group and these patients received interventions using their self-designed exercise diary for a total of 31 days. Their compliance with functional exercises before and after intervention and the incidences of venous thrombosis after intervention were compared between the two groups. Results After interventions, the compliance rate of arm exercises in the observation group was significantly higher than that of the control group (90.6% vs. 26.7%, P＜0.05). The incidence of venous thrombosis in the observation group was significantly lower than that of the control group (3.1% vs. 30.0% , P＜0.05). Conclusions Exercise diary can improve the arm exercise compliance of PICC patients and prevent the occurrence of venous thrombosis. It is worthy of clinical application and promotion.
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Objective:To investigate the expression of PKC? and TGF-? proteins in exocrine pancreatic carcinoma and their relationship. Methods: Forty-two specimens of pancreatic carcinoma and their adjacent tissues were examined for PKC? and TGF-? expression by tissue microarray technique and immunohistochemistry. Results: The expressions of TGF-?1 in the pancreatic carcinoma tissues and their adjacent tissue were 80. 9%(34/42) and 19. 1%(8/42), respectively(P
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Objective: To investigate the relationship between the Smad4-independent pathway of TGF-?1 and drug-resistance of pancreatic cancer. Methods: The sensitivities of Smad4 homozygous-deleted pancreatic cancer cell line BxPC3 to different kinds of anti-cancer drugs (5-Fu, Gemzar, Oxaliplatin, Cisplatin, CPT-11 and Epirubicin) were observed by MTT assay before and after they were transfected with full-length cDNA of TGF-?1 or treated with TGF-?1 (5 and 10 ng/ml) solution. Western blot was used to detect p170 protein expression after stimulation with different concentrations of TGF-?1. Results: Cisplatin had the most powerful killing effect on BxPC3 cells, followed by Oxaliplatin, 5-Fu and CPT-11 with moderate effect and Gemzar and Epirubicin with the least effect. Cells transfected with full-length cDNA of TGF-?1 or treated with TGF-?1 solution became less sensitive to Cisplatin. Western blot revealed upregulation of p170 expression by TGF-?1. Conclusion: The Smad4-independent pathway of TGF-?1 can increase the drug resistance of pancreatic cancer cells through upregulating expression of p170.
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Objective To establish a method for the determination of chimoni and forsythiaside in Kangbingdu Oral Liquid(KOL) and to develop a new quality standard for KOL. Methods A RP-HPLC method was adopted for the determination of chimonin and forsythiaside of KOL. The chromatographic conditions were as follows:Kromasil RP-C18(250 mm? 4.6 mm,5 ? m) column was used,a mixture of acetonitrile -0.1 % acetic acid(15 ∶ 85,v/v) served as mobile phase,the flow rate was 0.80 mL? min-1,and detective wavelengths of time process were 0→ 25 min:258 nm,and 25→ 30 min:277 nm. Theoretical plate number of chimoni was over 6000 and that of forsythiaside was over 5000. Results The chimonin showed a good linearity in the range of 48.8ng～ 1525 ng(r=0.999 4),and the linear range of forsythiaside was in the ranged of 56ng～ 1750 ng(r=0.999 5).The mean recovery of chimonin and forsythiaside was 97.65 % and 99.21 % respectively. The content of chimonin in the 10 batches of samples was in the range of 14.40～ 20.41 ? g? mL-1,and that of forsythiaside was in the range of 28.70～ 36.01 ? g? mL-1. Conclusion The developed method has been proven to be simple,stable and reproducible,and can be applied for quality control of the Kangbingdu Oral Liquid.