ABSTRACT
In order to study the damage mechanism of mineral dusts on the pulmonary alveolar macrophages (AM), the changes of their death ratio, malandialdthyde (MDA) content and activities of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) were measured. And the technique of cell culture in vitro was used to investigate the cytotoxicity of six mineral dusts (twelve crystal habits) from twelve mineral deposits. The results showed that wollstonite and clinoptilolite had no AM cytotoxicity while other fibrous and grainy mineral dusts could damage pulmonary AM in various degrees. The cytotoxicity of fibrous mineral dusts was greater than that of the grainy ones, and the cytotoxicity of dusts was positively correlated with the active OH- content in dusts, but not necessarily so with its SiO2 content. The high pH values produced by dust was unfavorable for the cells survival and the dusts with a low bio-resistance were safe for cells. The content of variable valence elements in dusts could influence their cytotoxicity and the surface charge of dusts was not a stable factor on their toxicity. It indicates that the shape of mineral dusts is one of the factors affecting cytotoxicity, and that the cytotoxicity of mineral dusts mainly depends on their properties.
Subject(s)
Animals , Male , Rabbits , Cell Death , Cells, Cultured , Dust , Macrophages, Alveolar , Cell Biology , Minerals , Chemistry , Toxicity , Toxicity TestsABSTRACT
AIM: To assess the role of surface free radicals and electromotive voltage of fibrous mineral dusts in rabbit pulmonary alveolar macrophage injuries induced by fibrous mineral dusts. METHODS: Changes in cell death ratio, malandialdehyde (MDA) and cellur electrophoresis ratio, lactate dehydrogenate (LDH)and superoxide dismitase(SOD) activities were determined, the technique of cell culture and Scanning electron Microscopy were used to examine the change of membranous permeability, charge and cellular shape. RESULTS: Fibrous wollastonite and tabulate clinoptilolite, which had no OH-, had no cytotoxicity, while fibrous sepiolite, fibrous palygorskite, fibrous brucite and chrysolite asbestos damaged pulmonary alveolar macrophages in various degrees because of the different OH- levels. All the six fibrous mineral dusts changed the cellular electrophoresis ratio. CONCLUSION: The surface electromotive voltage of fibrous mineral dusts is not an important factor, and the cytotoxicity of them may be related to OH- levels on the mineral dust surface.
ABSTRACT
Most solid materials in nature consist of minerals, they are ubiquitous on the surface of the earth. After inhaled in body, they will trigger pulmonary disease. The pathogenesis of mineral dusts is always in the way of disputing and consummating. Researcher should pay great attention to the adverse effect of mineral dust in nonprofessional environment. The biological action of mineral surface is an important aspect to discover mineral dust interface function and molecular toxicological mechanism. At present, the superfine mineral dust and nanometer suspending dust are the important components of dust environment safety assessment.