ABSTRACT
Antimicrobial peptides [AMPs] are extensive group of molecules that produced by variety tissues of invertebrate, plants, and animal species which play an important role in their immunity response. AMPs have different classifications such as; biosynthetic machines, biological sources, biological functions, molecular properties, covalent bonding patterns, three dimensional structures, and molecular targets. These molecules have multidimensional properties including antimicrobial activity, antiviral activity, antifungal activity, anti-parasite activity, biofilm control, antitumor activity, mitogens activity and linking innate to adaptive immunity that making them promising agents for therapeutic drugs. In spite of this advantage of AMPs, their clinical developments have some limitation for commercial development. But some of AMPs are under clinical trials for the therapeutic purpose such as diabetic foot ulcers, different bacterial infections and tissue damage. In this review, we emphasized on the source, structure, multidimensional properties, limitation and therapeutic applications of various antimicrobial peptides
Subject(s)
Anti-Bacterial Agents , InfectionsABSTRACT
As Pseudomonas aeruginosa is known the most common etiologic agent in microbial keratitis associated with contact lens use, this study was designed to study the distribution and patterns of resistance to antimicrobial agents of keratitis isolates in Iran. In this study, also the suitability of enterobacterial repetitive intergenic consensus [ERIC]-PCR to rapidly type P. aeruginosa strains isolated from patients with keratitis was examined. For this purpose, 57 clinically isolates of P. aeruginosa from keratitis patients referred to Farabi hospital were analyzed by antimicrobial susceptibility test using the disc diffusion method. Polymerase chain reaction with enterobacterial repetitive intergenic consensus primers [ERIC-PCR] was used to establish clonal relationship between the different isolates. All the strains showed resistance to at least 4 antibiotics, but all were susceptible to fluoroquinolones. Multidrug resistance was found in two isolates [3.5%] which were resistant to more than one category of antibiotics including aminoglycoside [gentamicin] and beta -lactam [cefazoline]. ERIC-PCR produced 53 different ERIC fingerprints, 49 of which contained only 1 strain. Eight of the isolates had 100% similarity, forming four real clones but considering 85% similarity cut off between isolates, 8 clones containing 25 isolates [43.8%] could be considered. Fluoroquinolones appeared to be the most effective agent against ocular P. aeruginosa isolates. Comparison of ERIC-PCR profiles revealed a low level of similarity among the strains analyzed. ERIC-PCR seems to be an inexpensive, fast, reproducible, and discriminatory DNA typing tool for effective epidemiologic surveillance of P. aeruginosa isolates potentially transmissible between patients with ocular infections