ABSTRACT
The p63 gene, a member of the p53 gene family, is expressed into at least six protein iso forms. The transcription factor p63 is a homologue of the tumor suppressor p53. Unlike p53, which is dispensable for normal development, p63 is critical for the development of stratified epithelial tissues such as epidermis, breast, and prostate. p63, is transcribed from two different promoters giving rise to two different proteins: p63, a member of the p53 family, is transcribed from two different promoters giving rise to two different proteins: TAp63 that contains the N-terminal transactivation domain and delta N that lacks this domain. p63 encodes multiple protein iso forms with both transactivating and transcriptional repressor activities that can regulate a wide spectrum of target genes. p63 is also implicated in tumor formation and progression in stratified epithelia, with evidence for both tumor suppressive and oncogenic properties
ABSTRACT
Embryonic stem cells [ESCs] are pluripotent, self-renewing cells. These cells can be used in applications such as cell therapy, drug discovery, disease modelling, and the study of cellular differentiation. In this experimental study embryonic stem cells cultured in the laboratory and were amplified. Total RNA was extracted from cells and converted to cDNA. The replication factor Oct3/4 gene was amplified by reverse transcription-polymerase chain reaction [RT-PCR] and cloned into the pTZ57R/T vector. Legated product had been transformed into susceptible bacteria and transformed bacteria were screened on a selective medium. Plasmids extracted from bacteria and enzyme digestion to confirm the sequencing was performed. Results of enzyme digestion were sequenced. Cloned gene can prepare a gene cassette to produce stem cells from somatic cell