Significance of albumin and C-reactive protein variations in 300 end stage renal disease patients in Tehran university of medical sciences hospitals during year 2010

Protein-energy malnutrition, wasting and inflammation are frequent complication among patients with end-stage renal disease [ESRD]. Malnutrition is associated with cardiac co-morbidity, inflammation and poor survival in ESRD patients. Serum albumin is a well-known marker of nutrition in ESRD patients. Serum albumin is still the most commonly used nutritional marker in ESRD patients. C-reactive protein [CRP], the major acute phase response [APR] protein is elevated in these patients. High CRP levels are linked to the degree of atherosclerosis in coronary, peripheral, and extracranial brain arteries. The aim of the present study was to investigate nutritional factor [albumin] and CRP levels in ESRD patients. In this cross-sectional study a total of 300 patients who had ESRD and had been on hemodialysis treatment for at least 6 months were selected. The laboratory tests consisted of measurement of CRP and albumin using high sensitive ELISA kits. The study patients included 157 males [52.3%] and 143 females [47.7%] with average age of 41.5 +/- 14.3 years. Mean CRP level was 7.96 mg/ dl [+/- 1.52], mean serum albumin was 4.07 g/dl [+/- 0.19].Of 300 patients, 21 died [7%]. These were patients with serum albumin<4 g/dl and CRP>9.5 mg/dl. This study showed that low albumin and high CRP levels are the main predictors for death. There was a significant difference between CRP and albumin levels in ESRD patients [P<0.0001]. Measuring CRP as a marker of inflammation can be helpful in managing these patients

Purification of alpha-amylase from bacillus subtilis line No 1024 with ATCC 465

Alpha-amylase E.C.3.2.1.1. [1,4 glucan, 4 glucanohydrolase] can be obtained from salivary glands, pancreas and microorganisms such as Pseudomonas and Asperigllus, as well as muscles and ovarian tubes. Alpha-amylase from Bacillus subtilis #1024[ATCC 465] was purified with a highest degree of purity in our laboratory [31.59 U/mg]. The extracellular alpha-amylase was subjected to different purification techniques such as anionic and cationic exchange chromatography and preparative electrophoresis. The final fold of purification was equivalent to 38, which was higher than the previous reported values, polyacrylamide gel electrophoresis with sodium dodecyl sulphate [PAGESDS], Laemmeli method, gave a molecular weight of 82000 Daltons