ABSTRACT
Background: N-acetyl cysteine [NAC] was proposed as an adjuvant to clomiphene citratefor ovulation induction in patients with polycystic ovary syndrome [PCOS] without clomiphene citrate resistance
Objective: to evaluate the effect of NAC on pregnancy rate in PCOS patients who were candidates for intrauterine insemination
Materials and Methods: in this randomized clinical trial 97 PCOS women aged 18-38 years were enrolled in two groups, randomly. For the case group [n=49], NAC [1.2 gr]+ clomiphene citrate [100 mg] + letrozole [5mg] were prescribed Daily from the third day of menstruation cycle for five days. The control group [n=48] had the same drug regimen without NAC. In order to follicular development, GONALF was injected on days of 7-11 menstrual cycles in all participants. When the follicle size was 18mm or more, HCG [10000 IU] was injected intramuscular and the intrauterine insemination was performed after 34-36 hr
Results: there were not significant differences between study groups regarding mean endometrial thickness [p=0.14], mean number of mature follicles [p=0.20] and the pregnancy rate [p=0.09]
Conclusion: NAC is ineffective in inducing or augmenting ovulation in PCOS patients who were candidates for intrauterine insemination and cannot be recommended as an adjuvant to CC in such patients
ABSTRACT
It has been demonstrated that insulin signaling pathway related genes have important roles in poly cystic ovary syndrome [PCOS] risk. The goal of present investigation was to assess the potential association between INSR/Nsil[rs2059806] and INSR/Pmll [rs1799817] SNPs and PCOS. 50 women with PCOS and 47 normal controls entered the study. Nsil and Pmll SNPs in the INSR gene were determined by RFLP-PCR. INSR/Nsii [rs2059806] SNP GG, GA, AA, G and A genotypic and allelic frequencies were 45[90%], 5[10%], 0[0%], 95[95%] and 5[5%] in cases and 41 [87.2%], 6[12.8%], 0[0%], 88[93.6%] and 6[6.38%] in controls, respectively. INSR/ Pmll [rs 1799817] SNPs resulted in three genotypes of CC, CT, and TT with C and T alleles. The frequencies of Pmll [rs 1799817] SNPs in the INSR gene were 37[37%] and 63[63%] in cases, also 39[41.49%] and 55 [58.51%] in controls regarding T and C alleles. The frequencies of Pmll [rs 1799817] SNPs in the INSR gene were 4[8%], 29[58%], and 17[34%] in cases, also 5[10.64%], 29[61.7%], and 13[27.66%] in controls regarding TT, TC, and CC genotypes, respectively. The present study as the first investigation of its own kind in Iranian Azeri Turkish women, reported no association between Nsil [rs2059806] and Pmll [rs 1799817] SNPs in the INSR gene and PCOS risk
ABSTRACT
Polycystic ovary syndrome [PCOS] is known as a metabolic disorder. The results of recent studies implied that vitamin D receptor [VDR] genetic variants may impact PCOS and insulin resistance in women with PCOS. The aim of the present study was to determine the VDR TaqI gene variant in exon 9 [T/C] [rs731236] in normal controls and patients with PCOS for the first time in Iranian Azeri women. In this case control study between April 2011 and June 2012, a total of 76 women aged 18-40 years [38 patients with PCOS and 38 healthy women as normal controls] participated. Genotypes of VDR TaqI in exon 9 [T/C] [rs731236] were determined using the PCR-RFLP method. The frequencies of VDR TaqI T anc C alleles were 0.605 and 0.395 in cases and 0.697 and 0.303 in controls. Also, the genotypic frequencies of VDR TaqI were 16] [42.11], 14[36.84], and 8[21.05] in cases, and 17[44.74], 19[50], and 2[5.26] in controls for TT, TC and CC genotypes respectively. There was no difference in genotype and allele frequencies between PCOS and controls [p value>0.05] with the exception of the CC genotype [p value=0.04]. This report, a first of its own kind in Iranian Azeri patients, suggests that the CC genotype of VDR TaqI in exon 9 [rs731236] is associated with PCOS