ABSTRACT
To find the synergistic effect between miconazole and griseofulvin on common human Candida. A serial dilution of miconazole and griseofulvin in Yeast Nitrogen Base [YNB] was prepared in test tubes. 5011 of standardized suspension of yeasts was inoculated into each test tube and incubated at 30°C for 24-48 hours. The antifungal activity of miconazole alone or with griseofulvin was measured in vitro against 300 isolates of the Candida. Miconazole and griseofulvin in combination showed a Minimum inhibitory Concentration [MIC] of 6.7mg/l miconazole [M] and 20mg/l griseofulvin [G] for C. albicans, 13mg/I M and 20mg/I G for C. tropicalis, 4.9mg/I M and 20mg/I G for C. pseudotropicalis and 14.2mg/I M and 20mg/I G for C. parapsilosis. The combination of miconazole with griseofulvin resulted in synergistic activity [Fractional Inhibitory Concentration, FIC <0.82] against C. albicans in a microdilution checkerboard assay. FICs for other species were as follow: FIC <0.75 for C. tropicalis, FIC <0.86 for C. pseudotropicalis and FIC <0.79 for C. parapsilosis. These results suggest that combination of griseofulvin and miconazole regimens may reduce usual dosage of miconazole and it is helpful in the control of infections caused by Candida species