ABSTRACT
Herbal medicines are still most popular, abundant and affordable remedies for curing various ailments. Garlina is one of the herbal formulations of Hamdard Laboratories [waqf] Pakistan used to treat cardiovascular diseases and elevated sugar level. However, there is no scientific data available regarding the potential toxicity. Therefore, the present study was to assess the acute and sub-chronic toxicity in rats. The single dose of Garlina 5000mg/kg were administered orally and observed for 14 days. A sub-chronic toxicity test was performed at 2000mg/kg of Garlina daily for 30 days. Control rats received saline. The biochemical, hematological and histopathological analysis was carried out. The acute toxicity LD50 was determined to be >5000mg/kg. The result of acute and sub-chronic toxicity revealed no mortality and sign of toxicity. Garlina did not elicit any significant change in body weight, hematological and histopathology analysis when compared to saline treated rats. The relative weight of organs was not affected by the treatment. While the daily dose of Garlina for humans is 20mg/kg. However, the sub-chronic toxicity at 2000mg/kg dose of Garlina exhibited significant increase in gamma glutamyltransferase while total protein significantly decreased. Results obtained from study demonstrated that there is wide margin of safety for the therapeutic use of Garlina and significant decrease in LDL, atherogenic index, GGT and bilirubin direct at the dose of 5000mg/kg further strengthen the use as hypolipidemic and hypoglycemic agent
ABSTRACT
The purpose of this study was to determine the safety profile of a polyherbal drug, Garlina. The acute toxicity was carried out at 5000 mg/kg administered orally while sub-chronic toxicity was assessed by daily oral dosing of 2000mg/kg in rabbits following ABPI and BTS guideline, 2009 and EMA, 2000, respectively. The outcomes of Garlina-treated group were compared with control group that received saline. The results of Garlina administered orally once at a dose of 5000 mg/kg and 2000 mg/kg/ day consecutively for a period of one month does not produce any remarkable adverse effects when analyzed histopathologically or biochemically. Similarly, hematological profile, body and organs weight also remained unchanged in its presence. However, a significant increase in uric acid at a single dose of 5000 mg/kg and a decline in RBCs count at the dose of 2000 mg/kg of Garlina were observed that will be monitored cautiously in the on-going clinical trials. The findings obtained in this study suggest that at prescribed dose Garlina is relatively safe for human consumption
ABSTRACT
The herbal drug aftimooni has been used for relief of anxiety and insomnia, however, no pharmacological studies have been reported. Therefore, the drug Aftimmoni was given orally to rats and mice to study its action on central nervous system. It is observed that aftimooni significantly potentiated pentobarbitone induced sleeping time at the dose of 1.070ml/kg [5 times greater dose than human dose]. Moreover, it significantly increased the rearing and number of square crossed in open field test. In elevated plus- maze, Aftimooni showed an anxiolytic effect by increasing the time spent in open arm. Additionally, a significant and dose dependent inhibition on writhing response induced by acetic acid was observed at the doses of 0.214ml/kg and 0.428ml/kg. However, Aftimooni did not affect immobility time in forced swimming test. These observations suggest that the drug Aftimooni possesses potential anxiolytic, hypnotic, exploratory and analgesic effects
Subject(s)
Animals, Laboratory , Ethnopharmacology , Anti-Anxiety Agents , Plants, Medicinal , Hypnotics and Sedatives , Analgesics , Rats, Sprague-DawleyABSTRACT
Aujaie is a herbal product used for the treatment of arthritic condition. An investigation has been carried out to determine its anti-inflammatory and antinociceptive activity on mice. The results show that Aujaie significantly and dose dependently inhibit carrageenan- induced paw oedema as well as acetic acid induced writhing response. The effects are comparable to Indomethacin