ABSTRACT
Distinction of hydatidiform moles [HMs] from non-molar abortions and sub-classification of HMs are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. The objective of this study was to determine the role of DNA flow cytometry in distinguishing molar from non-molar pregnancies. This retrospective study was conducted at the Department of Pathology, Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran, between 2006 and 2010. DNA ploidy analysis and histopathologic re-evaluation were performed on paraffin-embedded tissue from 36 [17 complete and 19 partial] molar and 24 hydropic abortus [HA] cases which were previously diagnosed based on histomor-phologic study. Of the 17 cases initially diagnosed as complete HM [CHM], 9 were dip-loid, 2 were triploid, 5 were tetraploid and 1 was aneuploid. Of the 19 initial partial HMs [PHMs], 2, 8, 1 and 8 cases were diploid, triploid, tetraploid and aneuploid, respectively. In the initial HA category [n=24], 14 diploid, 1 triploid, 5 tetraploid, and 4 aneuploid cases existed. Following flow cytometry and histopathologic re-evaluation, 1 case with previous diagnosis of HA was reclassified as PHM, 2 initial PHMs were reclassified as CHM and 2 initial CHMs were categorized as PHM. The results show that correct diagnosis of PMH is the main challenge in histological diagnosis of gestational trophoblastic disease [GTD]. DNA flow cytometric analysis could be an informative supplement to the histological interpretation of molar and hydropic placentas
ABSTRACT
Human Hydatid cyst is a wide spread disease. Its an endemic disease in Iran. It's caused by Echinococcus granulosus. Hydatid cyst although known to occur in most organs and body areas, is extremely rare in the female reproductive organs. This is a case report of ovarian Hydatid cyst in a 42-year-old woman. She presented with feeling of enlargement of abdomen and abdominal pain. On physical examination, a large mass in lower abdomen palpated. On imaging study, ultrasonography reported a large heterogeneous multicystic mass measuring 155 97 mm in pelvic cavity and hypo gastric area. The CT-scan with IV contrast reported a huge cystic lesion, containing multiple septi in pelvic cavity that could be due to ovarian lesion. After surgical procedure, histopathological examination showed typical laminated and germinative layers with hexagonal scolecies of Hydatid cyst. Since the common sites of hydaid cyst are liver and lung and the imaging studies are highly diagnostic for Hydatid cysts, this illustrated case is interesting for uncommon site, not suggested by imaging studies and with clinical [surgical] impression of malignant ovarian tumor