ABSTRACT
Periodontitis [PD] is known to be one of most prevalent worldwide chronic inflammatory diseases. There are several treatments including antibiotics for PD; however, since drug resistance is an increasing problem, new drugs particularly derived from plants with fewer side effects are required. The effects of trans-anethole on IL[-1] beta and TNF-alpha level in a rat model of PD were investigated and compared to ketoprofen. Eschericia coli lipopolysaccharide [LPS, 30 microg] was injected bilaterally into the palatal gingiva [3 microL/site] between the upper first and second molars every two days for 10 days in anesthetized rats. Administration of either trans-anethole [10 or 50 mg/Kg, i.p.] or ketoprofen [10 mg/Kg, i.p.] was started 20 minute before LPS injection and continued for 10 days. Then, IL[-1]beta and TNF-alpha levels were measured in blood samples by ELISA at day 0 [control] and at day 10. Anethole at both concentrations significantly suppressed IL[-1]beta and TNF-alpha production when compared to LPS-treated rats. The suppressive effects of anethole on LPS-induced pro-inflammatory cytokines were almost similar as seen with ketoprofen. In conclusion, the present results suggest that anethole may have a potent inhibitory effect on PD through suppression of pro-inflammatory molecules; therefore it could be a novel therapeutic strategy for PD
ABSTRACT
The main goal of this ex vivo study was to assess and compare the cellular and electrophysiological effects of two dental biomaterials, white mineral trioxide aggregate [WMTA] and calcium enriched mixture [CEM] cement, on neuronal cell excitability and electrical properties. A conventional intracellular current clamp technique was used to study the cellular effects of WMTA and CEM on the excitability, firing and the shape of action potential of neuronal soma membrane of F1 nerve cells. The dental biomaterials were prepared according to the manufacturers' directions and were applied to the bathing media and 0.05 mL of total mixture of each dental material at a distance of 3 mm from the cells. Findings indicated that exposure to both dental biomaterials shifted the irregular high frequency firing type observed in control conditions to a more regular low frequency firing pattern. Neuronal exposure to WMTA, but not CEM, significantly hyperpolarized the cell resting membrane potential. Both treatments significantly influenced the duration and the amplitude of action potentials. Extracellular application of either CEM or WMTA caused a significant increase in the after hyperpolarization [AHP] amplitude and AHP area, but the potentiating effect of WMTA was more effective than CEM. Treatment with WMTA or CEM resulted in a profound alteration in the firing behaviour of F1 cells and changed the AP characteristics. Both dental biomaterials reduced the neuronal activity possibly through enhancement of K[+] outward current. This may possibly explain the positive mechanisms of these biomaterials in regenerative endodontics, though further research is needed for such a conclusion
ABSTRACT
Mineral trioxide aggregate [MTA] is an endodontic material with different clinical applications e.g. root-end filling, pulp capping and perforation repair. It has been reported to possess antimicrobial and antifungal activities. The aim of this study was to examine the effect of White MTA on formalin-induced hyperalgesia in a rat with inflammatory pain. Inflammatory pain was induced by subcutaneous [SC] injection of formalin [40 microL, 2.5%] into the rat upper lip. The nociceptive behavioral responses i.e. shaking of the lower jaw and face rubbing were quantified. 40 uL of eugenol [50 mg/kg], WMTA [20 mg/0.2 mL] or ketoprofen were injected solely or in combination with formalin 2.5% and the behavioral responses were compared with those observed after formalin treatment alone. One-way ANOVA, Tukey were used for analysis of data. Formalin 2.5% provoked a biphasic nociceptive response, with an early and short lasting first tonic phase followed by a second phase. Solely SC injection of either WMTA or ketoprofen [a non steroidal anti-inflammatory drug] did not stimulate any significant nociceptive behaviour. However, injection of eugenol [a pain relieving agent] induced the early phase not the tonic phase of nociceptive response. WMTA, eugenol or ketoprofen injection 20 min before formalin injection attenuated the first phase but somehow prevented the induction of the second phase of nociceptive responses which were produced by formalin. Behavioural nociceptive responses including shaking of the lower jaw and face rubbing were significantly reduced when the subject was pretreated with either WMTA or ketoprofen [P<0.001]. In this study, WMTA induced pain reduction by suppression of the formalin-induced nociceptive response