ABSTRACT
Human serum albumin has been used as a model protein for protein folding and ligand binding studies over many decades. Due to its long life period and high concentration in plasma, HSA is highly sensitive to glycation. It is reported that 175 mg/dL glucose concentration is a threshold of kidney activity for the beginning of excretion of glucose. PH denaturation of HSA in absence and presence of different concentrations of glucose is studied and based on the Pace two-state model, the findings are analyzed. In addition, florescence emission data of albumin range in the period of 300-500 nm was depicted. The amounts of free energy change and [D] [1/2] parameters of unfolding in correspond to florescence date indicate that glucose induces fine structural change in human serum albumin. Results showed that 175 mg/dL glucose concentration is a critical point for albumin structural and functional alteration
ABSTRACT
Diabetes mellitus is a common name for a group of diseases of a much diversified etiopathogenesis, characterized by chronically increased concentration of blood glucose. Diabetes results from alterations of various genes, each having a partial and additive effect. The inheritance pattern is thus complex, and environmental factors play an important role in favoring or delaying the expression of the disease. Diabetes can cause devastating complications, including cardiovascular diseases, kidney failure, and blindness, leg and foot amputations, delay in wound healing, which often result in disability and death. Fibroblast cells play a critical role in wound healing. They are the most common cells of connective tissue in animals. Tissue damage stimulates fibrocystic and induces the mitosis of fibroblasts. Wound healing processes in diabetic patients are so longer and sometimes cause to cut damaged tissue. In this study Fibroblasts were isolated from foreskin and cultured as primary cell culture in different concentrations of glucose [8.8 mmol/l, 13.13 mmol/l, 18.31 mmol/l, 27.7 mmol/l, 37.18 mmol/l, 47.17 mmol/l, 83.24 mmol/l, 124.8 mmol/l and 166.4 mmol/l] for 48h incubation time. Traditionally, the determination of cell growth is done by counting viable cells after staining with a vital dye. Among several approaches have been used in the past, The MTT method of cell determination is most invaluable to cultures which are prepared in multiwall plates. This assay is a sensitive, quantitative and reliable colorimetric assay that measures viability, proliferation and activation of cells. The results of this preliminary study suggest that altered glucose concentrations may affect fibroblast behavior in ways that are important for tissue repair and wound healing. The cells had low level of confluency and viability and in high concentration fibroblast had very low cell division
ABSTRACT
Aldolase C as a glycolytic enzyme is associated with cellular structure at developmental stages of all cells, and this is particularly evident during the early stages of morphogenesis. It seems that expression of aldolase C can be regulated by the rate of differentiation that depends on the level of transcription or mRNA stability. There are several techniques to detect gene expression here proteomics was used for determining expression of aldolase C [as a differentiation factor] in several cell types and basal cell carcinoma [BCC] tissue. The human astrocytes were differentiated from mesenchymal stem cells, fibroblast cells were cultured as primary cell culture and BCC tissue was taken from the patient. The fibroblast cells divided into two groups including sham and exposed groups. The exposed cells are them that were exposed to continue Extremely Low-Frequency Electromagnetic Fields [ELF-EMF]. The analysis of 2DE gels, showed different expression of aldolase C in mentioned cells. The findings indicate that the amount of aldolase C expression decreases as differentiation process develops