ABSTRACT
Non-fermenting Gram-negative bacteria are unable to ferment sugars in order to generate energy. They are ubiquitous in nature, and have a wide geographic distribution. They are also common in hospital settings, and may be isolated from humidifiers, ventilator machines, dialysis machines and other equipment, as well as from the skin of hospital personnel. This study focused on the isolation of multidrug resistant [MDR] non-fermenting Gram negative bacteria from clinical samples. Antimicrobial susceptibility, detection of extended spectrum beta-lactamases [ESBL], and the presence of CTX-M and Metallo beta-lactamase [MBL] in the isolated bacteria were evaluated. Agar dilution method was used to test the susceptibility of the isolates to 10 antibacterial agents. All the isolates that were resistant to >/= 3 antibacterial agents from different classes were regarded as MDR [111 isolates] and were selected for further studies. beta-lactamase and ESBL production were detected by nitrocefin discs, combined discs [CD] and double discs plus CD [DCDT]. blaCTX-M and MBL were detected by PCR and EDTA synergy methods respectively. Among the MDR isolates the isolation frequency of Pseudomonas aeruoginosa, Stenotrophomonas maltophilia and Acinetoacter baumannii were 83.7%, 9.9% and 6.3% respectively. Resistance to imipenem [0.9%] and Ceftazidim [13.6%] was low, but resistance to other beta-lactams was high, and 29.7% were resistant to >/= 6 antibacterial agents from different classes simultaneously. beta-lactamase was produced by 41.4% of the MDR isolates. Detection of ESBLs by a CD [59.4%] or DCDT test [46.8%] was not significantly different, but with a combination of CD and DCDT a higher percentage of ESBLs in the isolates [P