ABSTRACT
The purpose of this study was to compare the subcutaneous tissue responses of freshly mixed or set endodontic root canal sealers [i.e. RoekoSeal, AH26, AH Plus] in Wistar Albino rats. Seventy-two male albino rats weighing 200-250g were used. The animals were randomly divided into six groups of 12 rats each. Root canal sealers were implanted in subcutaneous tissue in both freshly mixed and set conditions. The animals were sacrificed after 7, 14, and, 60 days. After histological preparation and Hematoxylin and Eosin [H and E] staining, the specimens were evaluated for capsule thickness, severity and extent of inflammation, and necrosis. Results were statistically analyzed using Multivariate ANOVA test. Differences between set and freshly mixed root canal sealers were significant [P=0.014], but not significant between test materials and controls, except for capsule thickness and extent of inflammation between control and AH26 [P=0.019 and P=0.006 respectively]. The interaction between the type of material and setting condition was significant for capsule thickness and severity of inflammation in AH26 specimens at 14 and 60 day [P=0.001]. Based on the results of this study assessing the biocompatibility, both set and freshly mixed states can be used
ABSTRACT
Biocompatibility is a desirable feature for root-end filling materials. In this study we aimed to compare a new material called cold ceramic [CC] with intermediate restorative material [IRM] and mineral trioxide aggregate [MTA] using Methyl-tetrazolium bromide [MTT] assay. The materials were tested in fresh and set states: [n=108]. The cytotoxicity was compared using L929 fibroblasts as an indicator; tested materials were eluted with culture medium according to ISO: 109935 standard. Distilled water and culture medium served as positive and negative controls, respectively [n=36]. The results were evaluated at 1, 24 hours and 7 days. Data were statistically analyzed by one-way ANOVA for each time interval and material status and t-tests. The cytotoxicity of the tested materials were statistically different at the various time intervals [P<0.001]. IRM was the most cytotoxic root-end filling material [P<0.001], MTA demonstrated the least cytotoxicity followed by CC. Despite displaying the greatest cytotoxicity, IRM is approved by the American Food and Drug Administration [PDA]. Cold ceramic had significantly lower cytotoxicity compared to IRM, in all but one subgroup. Further investigations are required to assess the clinical applicability of this novel material