Protein chromatographic study on adult ascaris lumbricoides, ascaris vitulorum and toxocara canis

Chromatographic analysis using Sephacryl S.300 was done on protein extract of adult Ascaris lumbricoides, A. vitulorum and Toxocara canis worms. Six fractions were separated from both Ascaris species and nine from T. canis. Fractions revealed a variety of molecular weights in relation to the three species of worms that ranged from 6,000 - 70,000 daltons. Crude extract of T. canis showed cross reaction with both species of Ascaris sera using double gel diffusion, while no reaction was obtained using a partially purified antigen