ABSTRACT
Objective: To find out whether Casein Kinase 2 [CK2] and Survivin co-express and correlate in prostate as well as in breast cancer
Study Design: A cross sectional analytical study
Place and Duration of Study: Study was done at Army Medical College and in collaboration with Armed Forces Institute of Pathology [AFIP]. The duration of this research was two years
Material and Methods: The research was authorized by the Ethical Committee of AFIP. CK2 expression was determined by immunohistochemistry in paraffin embedded tissues from established patients of prostate cancer [n=30] and breast adenocarcinoma [n=30]. Correlation of CK2 with Survivin was evaluated. Data were analyzed through SPSS version 20. For studying the correlation between the two proteins, pearson correlation coefficient was calculated. Data was considered at p-value
Results: An expressively strong and affirmative correlation was found among expression of total CK2 and total Survivin in invasive along with non-invasive cases of both prostate and breast cancers. A significantly strong and positive correlation was found between nuclear CK2 and Survivin expression in non-invasive cases of prostate cancer and invasive as well as non-invasive cases of breast cancer. A significantly strong and positive correlation was found only between cytoplasmic CK2 and Survivin expression in non-invasive cases of prostate cancer
Conclusion: CK2 and Survivin expressions have strong and positive correlation in both prostate and breast cancer particularly in non-invasive stage of the cancer
ABSTRACT
Dexamethasone [DEX] is a potent synthetic glucocorticoid. It is given to women at risk of delivering prematurely in order to promote maturation of fetal lungs
Objectives: To evaluate the effects of DEX given to the albino rats during different stages of intrauterine lung development on the postnatal alveolar diameter
Materials and Methods: 40 dams were randomly separated into five equal groups A, B, C, D and E. DEX [injection Decadron] was given in a dose of 0.2 mg/kg body weight to the dams of group B on 11, 12 and 13 gd [embryonic stage], to group C on 16, 17 and 18 gd [pseudoglandular stage], to group D on 19 and 20 gd [canalicular stage] and to group E on 21 gd [saccular stage]. Group A dams didn't recieve any drug. After normal delivery of the dams of all groups, the sample size was obtained by collecting 24 pups from each group which were labeled as B1, C1, D1 and E1 respectively. Pups taken from group A were considered as control A1. Pups of all groups were allowed to grow upto 28 postnatal day. Right lungs were collected from the pups of each group after proper fixation. Serial 3 micro m sections were cut and stained with hematoxylin/eosin stains for detailed histological study of alveoli
Results: The pups of group B1 showed results similar to those of control group. The pups of group C1 and E1 showed increased mean diameter of alveoli [P<0.001]. The pups of group D1 showed highest mean diameter of alveoli with P-value <0.001
Conclusion: DEX has direct influence on developing lungs especially alveolar diameter even by little dose administration in prenatal stages of lung development
ABSTRACT
Finasteride, a synthetic 4-azasteroid compound, is a specific inhibitor of steroid Type II 5 alpha-reductase, which belongs to one of a new class of drugs that is potentially useful in treating androgenetic alopecia [male pattern baldness], hirsutism and possibly acne
Objectives: To evaluate the effects of Finasteride on albino rat hepatocyte diameters
Materials and Methods: 52 male albino rats were randomly divided into two equal groups A [which was control] and B [which was experimental]. Group B animals were given Finasteride 80 mg/kg body weight between 10-11 am, [provided by FEROZESONS laboratories] dissolved in normal saline, for 30 days through naso-gastric tube. The animals of the control group were given only normal saline through naso-gastric tube for the same period of time. Twenty four hours after the cessation of treatment Livers were collected from the rats of both groups after dissection. 10% Formalin solution was used to fix the liver. 5 micro m serial sections of liver were cut and stained with haematoxylin/eosin stains for detailed histological study of hepatocytes
Results: It was observed that in experimental group the diameter of hepatocytes was increased as compared with control group [p-value <0.001]
Conclusion: Finasteride was responsible for mild hepatocellular injury showing increase in the diameter of the hepatocytes