ABSTRACT
Objective: To evaluate the relation between nephrin levels and preeclampsia severity by comparing serum and urine levels of nephrin in the severe and mild groups according to severity of associated intrauterine growth retardation [IUGR] development
Methods: A total of 150 patients who attended our ante-natal clinic [ANC] were included in this study. We had 5 groups; Group 1:30 patients with mild preeclampsia [MP] and normal fetal development [NFD], Group 2:30 patients with severe preeclampsia [SP] and NFD, Group 3: 30 patients with MP and IUGR, Group 4: 30 patients with SP and IUGR and Group 5: 30 volunteers who were normotensive and non-preeclamptic. We obtained both blood and urine samples for measuring nephrin levels
Results: Both serum and urine nephrin levels were significantly higher for the fourth group compared with all other groups [p<0.001]. The levels of SP group with NFD were measured considerably higher than MP group out of IUGR and control group [p<0.001]. Urine and serum nephrin levels with gestational age of delivery showed a negative correlation [r=-0.621, p<0.001] and also urine and serum nephrin levels with birth weight showed a negative correlation too [r=-0.655 p<0.001]
Conclusion: Both serum and urine nephrin levels correlated with the severity of preeclampsia and IUGR development
ABSTRACT
To investigate if free PSA [fPSA] and total PSA [tPSA] values obtained from simultaneously collected urine, fresh and dried on filter paper, reflect the serum free and total PSA. The sera and 20 cc first voided urine from 33 consecutive men aged between 40 and 84 [mean 61 +/- 12], were collected in the morning and delivered to the laboratory. Three different aliquots of 100 microgram urine were taken with automatic pipette and dropped on 3 certain areas of a filter paper and allowed to dry for each patient. On each paper, borders of dried urine were marked. PSA values were obtained from the sera and fresh urine samples and recorded. Later on particular days dried urine samples were dissolved and eventually PSA values were derived and recorded again. The results were compared to each other. Correlations were evaluated by using an SPSS statistics program. Serum PSA values correlated weakly [r <0.24] with fresh and dried urine PSA values. While PSA in fresh and dried urine samples showed strong correlation [0.5< r < 0.74], a very strong correlation [r >0.75] among PSA values of dried urine samples of 1-day, 7- and 28-days, were seen. We conclude that PSA values obtained from fresh and dried urine could not reflect serum PSA values. But, because dried urine on a filter paper can be stable for years, it could be used for forensic purposes