Laudanosine detection after prolonged continuous infusion of atracurium

In the present study, atracurium was given by continuous intra venous infusion to twenty one patients classified into two groups: group I [10 patients] undergoing prolonged surgery and group II [11 patients, admitted to the ICU and requiring neuromuscular blockade to allow controlled mechanical ventilation. Plasma laudanosine the metabolic of acracurium level was estimated by HPLC in both groups being 1.92 mg/ml and 1.91 mg/ml respectively, far away from toxic level recorded in experimental animals. Measurement of blood histamine level by fluorometric assay revealed insignificant changes during atracurium infusion Clinical assessment of patients of both groups demonstrated no sign, of cerebral irritation or convulsions as well as no evidence of cardio vascular instability. In conclusion, prolonged infusion of atracurium fo-surgical procedures and in intensive care even for seriously ill patient, is potentially safe

Pathobiochemical studies on the effect of cytoxan, 5- fluorouracil and methotrexate [CMF] on the liver of mice

The effect of Cyclophosphamide, Methotrexate and Fluorouracil [CMF] on the liver enzymes and liver histopathology was investigated in experimental mice. The drugs were administered on single drug bases and in combination in two schedules, one-day pulse and/or once every other day for 5 doses. The toxicity was measured by determination of serum liver enzymes [5-Nucleotidase, Alkaline phosphatase and glucose-6-phosphatase]. Histopathological examination of mice liver showed that the initial changes that were observed by one day pulse administration was stronger than that observed after repeated small doses. However, the rate of recovery was much rapid after single administration. Among the enzymes tested it seems that serum alkaline phosphatase was the most sensetive one that reflects the pathological changes of the liver

Pathobiochemical studies on the effect of cytoxan, 5- fluorouracil and methotrexate [CMF] on mice intestine

The effect of cyclophosphamide, Methotrexate and Fluorouracil [CMF] was investigated on intestinal enzymes [5-nucleotidase, Alkaline phosphatase and Glucose-6-phosphatase] as well as on the histopathology of intestinal mucosa of mice. The drugs were given either as single agent or in combination in two dose schedule, a one day puls therapy or once every other day for 5 doses. Intestinal 5-nucleotidase is the most significantly elevated enzyme and no sign of recovery was observed 10 days after [CMF] administration. Histopathology of mice intestine showed that the initial changes that were observed by repeated small doses were more severe than those observed after single pulse administration, recovery of which needs more than ten days