ABSTRACT
Chitosan were prepared from cuticle of Lucilia cuprina maggots with two steps; deproteinization and deacetylation. It was characterized with solubility and Fourier Transform Infrared spectroscopy [FT-IR]. Chitosan was ball-milled to obtain the chitosan nanoparticles which characterized with dynamic light scattering [DLS] and transmission electron microscope [TEM]. Chitosan nanoparticles with degree of deacetylation [DDA] 80.5% were showed antibacterial activities against Klebsiella pneumoniae and Bacillus subtilis. The mode of action of chitosan nanoparticles on the tested bacteria was studied by TEM. Leakage of some cell contents, cell deformation mi rupture of cell were observed, therefore, the chitosan nanoparticles were observed to be a powerful antibacterial agent
ABSTRACT
Chitosan nanoparticles were studied as antimicrobial agent. The antibacterial activity of chitosan nanoparticles were investigated against three Gram-negative bacteria; Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi, and three Gram-positive bacteria; Staphylococus aureus, Enterococcus faecalis and Streptococcus pyogenes. The antifungal activity were examined against three fungi; Geotrichum candidum, Candida krusei and Candida parapsilosis. The antiviral activities were tested against three viruses; Rift Valley Fever [RVFV], Herpes simplex-1 [HSV-1] and Coxsackie viruses. Chitosan nanoparticles were inhibited all bacteria and fungi except E.faecalis seemed to be resistant strain. Infectivity titers of all viruses were reduced by chitosan nanoparticles, which are a natural antimicrobial agent