Loss of p16 protein expression and its association with epstein-barr virus lmp-1 expression in hodgkin's lymphoma

Expression of Epstein-Barr virus Latent Member Protein-1 [EBV LMP- 1] and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma. Sections were made from archival formalin-fixed and paraffin embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies. In Hodgkin's Lymphoma [HL], the loss of P16 was higher in LMP-1 positive cases [61%] than LMP-1 negative cases [25%; P=0.072]. Conversely, in Non- Hodgkin's Lymphoma [NHL], none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male [75%] than female [33%]. Also, there was a significantly higher proportion of LMP-1 positive cases showing loss of P16 in HL [11: 18], compared to those in NHL [0: 8, P<0.001], confirming a difference between HL and NHL, concerning the LMP-1/P16 relationship. A trend for an association between loss of P16 and LMP-1 expression was observed in HL but not NHL patients. These findings suggest that there are molecular and clinical differences in the pathogenesis and development of different subtypes of lymphoma

Hepatoprotetive, cardioprotective and nephroprotective actions of essential oil extract of artemisia sieberi in alloxan induced diabetic rats

The aim of the current study is to evaluate the potential mechanism of antidiabetic action of the essential oil of Artemisia sieberi and its effects on some hematological and biochemical parameters in alloxan induced diabetic rats. Extraction of the essential oil from aerial parts of A. sieberi was preformed by hydrodistillation. Fifty rats were divided into five groups. Groups I and II normal rats given 1 mL/day of dimethyl sulfoxide and 80 mg/kg BW of this oil extract, respectively. Groups III, IV and V diabetic rats given 1 mL/day of dimethyl sulfoxide, oil extract [80 mg/kg BW] and metformin [14.2 mg/kg BW], respectively. Several hematological and biochemical parameters were assessed. Oral administration of the extract resulted in a significant reduction in the mean values of blood glucose, glucagon, cholesterol, triglyceride, LDL-C, ESR, urea, uric acid, creatinine accompanied by an increase in the mean values of the total protein, albumin, insulin, HDL-C, neutrophile count and PCV in diabetic rats. No significant changes in these parameters were found in the control group. The effects produced by this extract were closely similar to a standard antidiabetic drug, metformin. In conclusion, the present study indicates that the essential oil extract of A. sieberi appears to exhibit cardioprotective, nephroprotective and hepatoprotective activities in alloxan induced diabetic rats