Spectrophotometric determination of Cu[2+] and monitoring of Hg[2+] and Ni[2+] in some Iranian vegetables using 6-[2-naphthyl]-2, 3-dihydro-as-triazine-3-thione

Recently, 6-[2-naphthyl]-2, 3-dihydro-as-triazine-3-thione [NDTT] was synthesized in laboratory and used successfully for the spectrophotometric determination of nanogram levels of Cu[2+] in aqueous solution. This reagent forms a specific red complex with Cu[2+] ions after the extraction by chloroform at alkaline ph. The absorption of the complex in the UV region [313 nm] is about 8 times as strong as in the visible one [510 nm]. Mercury and nickel ions form yellow complexes with NDTT under the same conditions which interfere in the UV region and without effect on Cu [II] absorbance in the visible region. The studied vegetables include Mentha pipereta L., Anethum graveolens L., Beta vulgaris L., Coriandrum sativum, Petroselinum hortense H., Ocimum basilicum L., Spinacia oleracea L., Lactuca sativa L., and Brassica oleracea L

Preparation of 16-dehydropregnenolone acetate from diosgenin extracted from Iranian trigonella seeds

Trigonella foenum-graecum L., is a native plant which abundantly grows in Iran. Seeds of this plant contain the saponin diosgenin among other steroidal compounds. The total steroids of the dried seeds were extracted by ethanol [96%]. Column chromatography followed by crystallization were applicable to obtain diosgenin in pure form [0.15%]. The early method for disclosure of diosgenin ring F by acetic anhydride at 200°C and under pressure was avoidable under Lewis acids catalysis. Oxidation of the reaction product by CrO[3] followed by refluxing with acetic anhydride afforded 16-dehydropregnenolone acetate. LJV, IR, [1]H-NMR and mass spectrum were applied to identify and confirm the structures of diosgenin and 16-dehydropregnenolone acetate

Synthesis of 6- [2-Naphthyl] -2,3-dihydro-as-Triazine-3-thione as a sensitive reagent for the spectrophotometric determination of Cu [II]

A Spectrophotometric method for determination of Cu [II] based on the complex formation with a new reagent 6-[2-naphthyl]-2,3-dihydro-as-triazine-3-thione[NDTT] is described. NDTT was synthesized based on the knowledge available for the preparation of 6-phenyl-2,3-dihydro-as-triazine-3-thione [PDTT]. Reaction of 2-acetylnaphthalene 1 with amyl nitrite gave 2-naphthylglyoxal aldoxime 2, which upon reaction with thiosemicarbazide yielded 6-[2-naphthyl]-2,3-dihydro-as-triazine-3-thione [NDTT] 3. NDTT produces a red complex with copper which is easily extractable with chloroform at pH>8 while the reagent is not extracted under these conditions. The absorption of the complex in the UV region [313 nm] is about 7 times greater than in the visible region [508 nm]. The mole ratio of the complex which is formed between Cu [II] and NDTT is 2:3, which was calculated by both the mole ratio and the continuous variation methods. The absorbance of the complex obeys Beer's law in the concentration range of 0.08-2 micro g Cu[II]/ml chloroform with r = 0.998 and detection limit of 13 ng/ml. This procedure can be carried out in the presence of many cations and anions in the presence or absence of the masking agents

Process for isolating and purifying stigmasterol and sitosterol derived products from glycine max seeds

Two major sterol dos soybean, Glycine max L. [Leguminosae], were isolated with Thin Layer Chromatography [TLC] and argentic TLC. They were examined using NMR, IR and MS instrumental analysis and identified as stigmasterol [stigmast-5, 22-dien-3beta-ol] acetate and sitosterol [stigmast-5-en-3beta-ol] acetate