ABSTRACT
Objectives: To investigate whether remote ischemic conditioning (RIC) applied to patients with ST-segment elevation myocardial infarction (STEMI) before percutaneous coronary intervention (PCI) could affect circulating miR-208b level or not. Methods:Patients diagnosed with STEMI undergoing PCI from January 2016 to July 2017 were enrolled from the Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University.The patients were randomly allocated to two groups: (1) control group (n=25), PCI alone; (2) RIC group (n=50), PCI combined with RIC (three cycles of 5 min inflation and 5 min deflation of the right lower limb with blood pressure cuff performed before reperfusion). Serum miR-208b was measured before and immediately, at 24 h, and 48 h after PCI with real-time quantitative polymerase chain reaction. Results: The expression of miR-208b was significantly higher immediately post PCI than that before operation in the control group (84.1±9.0 vs 77.8±9.4; P=0.032), while it was significantly lower immediately post PCI than that before operationin RIC group (71.0±9.3 vs 77.4±8.8; P=0.028).miR-208b level was similar before PCI between the control and RIC groups (P=0.874), which was significantly reduced immediately post PCI in RIC group as compared with the control group (P=0.021).The peak value of creatine kinase isoenzyme (CK-MB) in the limb RIC group was significantly lower than that in the control group ([135.2±18.6] U/L vs [167.7±17.2] U/L; P=0.038).The area under the CK-MB curve of the RIC group was significantly smaller than that of the control group ([3 060.7±17.1] U/L vs [3 635.9±15.1] U/L); P=0.047]. The left ventricular ejection fraction (LVEF) in RIC group was significantly higher than that in the control group ([57.8±7.8]% vs [51.9±7.9]%; P=0.003) post PCI. The expression level of serum miR-208b was positively correlated with CK-MB AUC in RIC group (r=0.498, P<0.001). Conclusions: RIC of the lower limb prior to PCI could reduce miR-208b level and improve cardiac functionin STEMI patients.
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Objective To observe the clinical effect of oral administration of Fufangejiao liquid after drug-induced abortion. Methods A total of 120 women within 7 weeks' of gestation, who needed to terminate pregnancy and asked for drug-induced abortion, were evenly randomized into two groups: experimental group and control group. The experimental group was given Fufangejiao liquid (20ml, tid, for 7 days) after the drug-induced abortion; the control group was given Chanfukang particles (lOg, tid, for 7 days) after drug-induced abortion. The drug-induced abortion and amount of bleeding were observed in the two groups. Results (1) The complete abortion rate was 90. 0%(54 cases) in the experimental group and 86. 7% (52 cases) in the control group. (2) There were 88. 9%(48/ 54 cases) of the patients in the experimental group and 76. 9% (40/52) in the control group had a similar bleeding amounts to the usual menstrual amount. There were 9. 3%(5 cases) of the patients in the experimental group and 19. 2%(10 cases) in the control group had more bleeding (≤1 fold) than the usual menstrual amount(P<0. 05). (3)The days of bleeding, bleeding amount, and menstrual onset were similar in the two groups. Conclusion Administration of Fufangejiao liquid after drug-induced abortion can reduce the amount of bleeding after abortion and is therefore worth popularizing.
ABSTRACT
<p><b>OBJECTIVE</b>To establish the validation method and criteria for counting bacteria and fungi in microbial limit test which is described in the Pharmacopeia of China (ChP) 2005.</p><p><b>METHOD</b>According to the method set up for validation, the tested microorganisms with known counts were added to samples followed by the determination of the recovery.</p><p><b>RESULT</b>With different preparing method for testing samples, the recoveries for the tested microorganisms in testing samples were found to be over 70%.</p><p><b>CONCLUSION</b>Validation method for counting contaminated bacteria and fungi in drugs is recommended to follow the method established in this paper. The recovery for tested microorganisms should be not less than 70%.</p>