ABSTRACT
OBJECTIVES: Treatments for injured articular cartilage have not advanced to the point that efficient regeneration is possible. However, there has been an increase in the use of platelet-rich plasma for the treatment of several orthopedic disorders, including chondral injuries. Our hypothesis is that the treatment of chondral injuries with platelet gel results in higher-quality repair tissue after 180 days compared with chondral injuries not treated with gel. METHODS: A controlled experimental laboratory study was performed on 30 male rabbits to evaluate osteochondral injury repair after treatment with or without platelet gel. Osteochondral injuries were surgically induced in both knees of each rabbit at the medial femoral condyle. The left knee injury was filled with the platelet gel, and the right knee was not treated. Microscopic analysis of both knee samples was performed after 180 days using a histological grading scale. RESULTS: The only histological evaluation criterion that was not significantly different between treatments was metachromasia. The group that was treated with platelet gel exhibited superior results in all other criteria (cell morphology, surface regularity, chondral thickness and repair tissue integration) and in the total score. CONCLUSION: The repair tissue was histologically superior after 180 days in the study group treated with platelet gel compared with the group of untreated injuries. .
Subject(s)
Animals , Male , Rabbits , Cartilage, Articular/injuries , Knee Injuries/therapy , Platelet-Rich Plasma , Cartilage, Articular/pathology , Disease Models, Animal , Gels/therapeutic use , Knee Injuries/pathology , Reproducibility of Results , Time Factors , Treatment Outcome , Wound HealingABSTRACT
OBJETIVO: Relacionar a área de secção inicial do enxerto com o resultado da cirurgia de reconstrução do LCA. Foram operados oito cães, divididos em dois grupos, de acordo com o tamanho do enxerto: grupo A - 25% e grupo B - 40% da largura do ligamento patelar (LP). MÉTODOS: Após oito meses, os cães foram sacrificados para análise macroscópica e histológica dos ligamentos reconstruídos, utilizando-se o joelho contralateral do cão como controle. RESULTADOS: Em ambos os grupos, todos os ligamentos reconstruídos apresentaram-se viáveis e hipertrofiados; o enxerto de LP teve sua morfologia alterada, verificada através da medida do crimp e da celularidade, assemelhando-se com a do LCA. CONCLUSÃO: A área de secção do enxerto não influenciou o resultado histológico da cirurgia de reconstrução do LCA em cães.
OBJECTIVE: To correlate the initial grafting section area with the outcomes from anterior cruciate ligament (ACL) reconstruction surgery. Eight dogs underwent operations, divided into two groups according to graft size: Group A, 25% and Group B, 40% of the patellar ligament (PL) width. METHODS: After eight months, the dogs were sacrificed for macroscopic and histological analysis on the reconstructed ligaments. Each dog's contralateral knee was used as a control. RESULTS: In both groups, all the reconstructed ligaments were seen to be viable and hypertrophied. The morphology of the PL grafting had changed, which was observed by measuring the crimp and cellularity, and it resembled that of the ACL. CONCLUSION: The grafting section area did not influence the histological outcomes from ACL reconstruction surgery in dogs.