ABSTRACT
Objective@#To understand the prevalence of eating behavior problems among preschool children in rural Hunan, and to explore its determinants, so as to provide a reference for solving their eating behavior problems.@*Methods@#A multistage stratified cluster sampling method was used to randomly select 3 288 preschool children aged 3-6 years and their caregivers from 24 kindergartens between August and November 2019. Eating behavior problems questionnaire survey and peripheral blood hemoglobin detection were conducted among the preschool children, and unconditional Logistic regression model was used to analyze the determinants.@*Results@#The total prevalence of eating behavior problems among preschool children in rural Hunan was 66.8%(2 195/3 288), and the three most common eating behavior problems were poor appetite(40.1%, 1 317/3 288), picky eating(43.6%, 1 434/3 288), poor eating habits(44.4%, 1 460/3 288), respectively. The risk factors for general eating behavior problems included left-behind children, caregiver occupation as professional and technical staffs, average daily static activity time≥3 hours, anemia, and second-hand smoke exposure, and older age and daily moderate/high intensity activities were protective factors. In the analysis of the three most common eating behavior problems: The risk factors for poor appetite included left-behind children, anemia, and second-hand smoke exposure, and daily moderate/high intensity activities was protective factors. The risk factors for picky eating included left-behind children, caregiver occupation as professional and technical staffs, high family income, average daily static activity time≥3 hours, and daily moderate/high intensity activities was protective factors. The risk factors for poor eating habits included left-behind children, caregiver occupation as professional and technical staffs, average daily static activity time≥3 hours, and older age and daily moderate/high intensity activities was protective factors.@*Conclusion@#The prevalence of eating behavior problems among preschool children in rural Hunan was high, which is influenced by age, left-behind, caregivers occupation, family income, activities, anemia, and second-hand smoke exposure.
ABSTRACT
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23 CFU for pure culture, whereas 2.3 × 104 or 2.3 × 106 CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3 × 106 CFU, but PCR was negative at the level of 2.3 × 107 CFU. After simulated spleen and lung samples were treated with magnetic beads, the sensitivity of LAMP or PCR was 2.3 × 103 or 2.3 × 106 CFU, whereas 2.3 × 105 or 2.3 × 107 CFU for magnetic bead-treated liver samples. These results indicated that some components in the tissues could inhibit LAMP and PCR, and liver tissue samples had a stronger inhibition to LAMP and PCR than spleen and lung tissue samples. LAMP has a higher sensitivity than PCR, and magnetic bead capture of DNAs could remarkably increase the sensitivity of LAMP. LAMP is a simple, rapid and sensitive assay suitable for application in the field or poverty areas.