ABSTRACT
To study the coumarins of Anemone raddeana Regel, the compounds were separated by silica gel column chromatography and HPLC. Their structures were identified by their physicochemical property and spectral analysis. Two new compounds were isolated and identified as 4, 7-dimethoxyl-5-methyl-6-hydroxy coumarin (1) and 4, 7-dimethoxyl-5-formyl-6-hydroxycoumarin (2). The bioassays indicated that compounds 1 and 2 could significantly inhibit the proliferation of cancer cell, and showed the agonist effect on the transactivity of retinoic acid receptor-alpha (RARalpha). In addition, the two compounds had inhibitory effect against human leukocyte elastase (HLE).
Subject(s)
Humans , Anemone , Chemistry , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Cell Line, Tumor , Cell Proliferation , Coumarins , Chemistry , Pharmacology , Inhibitory Concentration 50 , Leukocyte Elastase , Metabolism , Molecular Structure , Plants, Medicinal , Chemistry , Receptors, Retinoic Acid , Genetics , Metabolism , Retinoic Acid Receptor alpha , Rhizome , Chemistry , Transcriptional ActivationABSTRACT
OBJECTIVE: To investigate the chemical constituents of Zornia diphylla (L.) Pers. METHODS: were isolated by chromatography on silica gel, Sephdex LH20, ODS C-18 and HPLC, and their structures were elucidated on the basis of spectral analysis. MTT assay in vitro was used to explore the inhibiting effect of the compounds on TE13 cancer cells and B16 cancer cells. RESULTS: Six compounds were isolated and identified as 7, 4'-dimethoxy isoflavone (1); 7-hydroxy-4'-methoxyisoflavone (2); 7, 3'-dihydroxy-4'-methoxyisoflavone (3); 7, 8-dihydroxy-4'-methoxyisoflavone (4); 7, 4'-dihydroxy-8-methoxyisoflavone (5); and 7, 4'-dihydroxy-isoflavone(6). All compounds showed anti-tumor activities against the cancer cells. CONCLUSION: All compounds are isolated from Zornia diphylla (L.) Pers. for the first time. Compounds 1-6 show inhibitory activity on TE13 cancer cells, and compound 3 shows inhibitory activity on B16 cancer cells. Copyright 2012 by the Chinese Pharmaceutical Association.
ABSTRACT
To study chemical constituents of Polygonatum odoratum (Mill.) Druce, the compounds were separated with column chromatography and HPLC. On the basis of physicochemical properties and spectral data, their structures were confirmed. Nine compounds were isolated and identified as 5,7-dihydroxy-6-methoxyl-8-methyl-3-(2',4'-dihydroxybenzyl)chroman-4-one (1), 5,7-dihydroxy-6-methyl-3-(2',4'-dihydroxybenzyl)chroman-4-one (2), 5,7-dihydroxy-6-methoxyl-8-methyl-3-(4'-methoxybenzyl)chroman-4-one (3), disporopsin (4), chrysoeriol (5), 5,4'-dihydroxy-7-methoxy-6-methylflavone (6), N-trans-feruloyltyramine (7), N-trans-feruloyloctopamine (8), and (+)-syringaresinol (9). Compounds 1-3 are new homoisoflavanones. Compounds 4-9 are isolated from this plant for the first time.