Correlation among ambulatory blood pressure indexes and P wave dispersion in patients with hyperten-sion / 心血管康复医学杂志

Objective :To explore P wave dispersion (Pd) change in patients with hypertension ,and study correlation among ambulatory blood pressure indexes and Pd .Methods : A total of 100 patients with hypertension treated in our hospital from Jul 2015 to Jul 2017 were selected as hypertension group .Another 76 healthy subjects without cardio-vascular diseases undergoing physical examination during the same period were selected as normal control group .Ac-cording to circadian rhythm of blood pressure ,hypertension group was further divided into dipper group (n＝36 ) and non-dipper group (n＝64).All subjects received 24h ambulatory blood pressure monitoring and 12-lead ECG .P wave maximum time limit (Pmax) ,P wave minimum time limit (Pmin) and Pd were compared among all groups ,and correlation among ambulatory blood pressure indexes and Pd were analyzed .Results : Compared with normal con-trol group ,there were significant rise in Pmax [ (103.13 ± 9.19) ms vs.(118.04 ± 11.14) ms] and Pd [ (24.62 ± 5.96) ms vs.(38.05 ± 7.76) ms] in hypertension group , P＝0.001 both .Compared with dipper group ,there were significant rise in Pmax[ (116.04 ± 10.17) ms vs.(121.13 ± 12.06) ms] and Pd [ (35.05 ± 8.01) ms vs.(40.05 ± 7.16) ms] in non-dipper group , P＝ 0.035 ,0.002. Pearson correlation analysis indicated that Pd was significant positively correlated with 24h mean systolic blood pressure (SBP) ,24h SBP standard deviation (SD) ,24h diastolic blood pressure SD ,SBP load ,daytime mean SBP and nighttime mean SBP (r＝0.291～0.455 ,P＜0.05 or ＜0.01). Conclusion : SBP ,SBP load and blood pressure SD are significant positively correlated with Pd in patients with hy-pertension ,suggesting that these indexes possess higher value in assessing atrial injury in these patients .

HPV infection of the external genitalia in men whose female partners have cervical HPV infection / 中华男科学杂志

<p><b>Objective</b>Human papilloma virus (HPV) is a necessary cause of cervical cancer and is also closely related to penile cancer, oropharyngeal cancer, and anal cancer in males. However, few studies are reported on male HPV. This study aimed to investigate HPV infection of the external genitalia in men whose female partners have cervical HPV infection.</p><p><b>METHODS</b>We collected the relevant data on the male outpatients whose partners had cervical HPV infection in our Department of Urology and Andrology from August to December 2016. We obtained samples with nylon swabs from the glans penis, corona, inner layer of the prepuce and penile body and detected different types of HPV infection using the Hybribio HPV typing kit, PCR and membrane hybridization.</p><p><b>RESULTS</b>Valid data were collected from 140 males, which showed 83.5% of HPV infection of the external genitalia, including 60 cases of HPV6 (43.2%), 27 cases of HPV16 (19.4%), 14 cases of HPV39 (10.1%), 13 cases of HPV18 (9.4%), 13 cases of HPV58 (9.4%), and 13 cases of HPV52 (9.4%). Redundant prepuce was found in 75.5% of the males, but there was no statistically significant difference in the incidence rate of HPV infection between the normal and redundant prepuce groups (P > 0.05).</p><p><b>CONCLUSIONS</b>Men who have the female partners with positive cervical HPV are at high risk of HPV infection and therefore need to be screened and treated so as to reduce HPV infection in both sexes.</p>

Relationship of abnormal sperm DNA methylation with early spontaneous abortion / 中华男科学杂志

<p><b>Objective</b>To investigate the relationship between the abnormal sperm DNA methylation level and early spontaneous abortion.</p><p><b>METHODS</b>We randomly selected 98 males who met the inclusion criteria and whose wives suffered from unexplained abortion or embryo abortion, and included another 46 normal healthy men present for pre-pregnancy check-up as controls. We examined the semen quality and sperm morphology, obtained the sperm DNA fragmentation index (DFI) by modified sperm chromatin dispersion, and measured the sperm DNA methylation level using the methylated DNA quantification kit and the colorimetric method.</p><p><b>RESULTS</b>Compared with the normal controls, the men in the unexplained abortion group showed a significantly lower rate of big-halo sperm (［45.50 ± 26.27］ vs ［36.49 ± 23.06］%, P = 0.038), a higher rate of abnormal-head sperm (［77.08± 12.21］ vs ［81.09± 10.89］%, P = 0.049), and a lower level of sperm DNA methylation (［0.47 ± 0.33］ vs ［0.36 ± 0.26］ ng/μl, P = 0.035). The sperm DNA methylation level was positively correlated with the percentage of big-halo sperm (OR=0.546, P<0.01). Multivariate regression analysis manifested that sperm head abnormality was an independent risk factor of early spontaneous abortion or embryo abortion (OR=1.032, P = 0.049), while the high methylation level was protective factor against early spontaneous abortion or embryo abortion (OR=0.244, P = 0.03).</p><p><b>CONCLUSIONS</b>The abnormal level of sperm DNA methylation may be one of the important reasons for early spontaneous abortion or embryo abortion.</p>

Semen quality and its influencing factors in preconception males in Nanjing area / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the semen quality and its influencing factors in preconception males in Nanjing area so as to provide some evidence for working out effective intervention measures.</p><p><b>METHODS</b>Totally 687 men receiving preconceptional physical examination were enrolled in this study. A questionnaire survey was conducted among the subjects along with an analysis of their semen quality.</p><p><b>RESULTS</b>The median of sperm concentration was 63.3 x 10(6)/ml (95% CI [19.88-119] x 10(6)/ml). The median of grade a sperm was 33.03% (95% CI [19.38-55.05]%), that of grade a + b sperm was 52.08% (95% CI [39.53-69.37]%), and that of teratosperm was 91.75% (95% CI [69-100]%). The median concentration of seminal plasma PMN-elastase was 195.55 ng/ml (95% CI [76.16-3330.38] ng/ml) and that of seminal plasma zinc was 7.62 μmol/L (95% CI [1.5-23, 45] μmol/L). The positive rates of Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), and Gardnerella vaginalis (GV) were 42.4%, 0.3%, and 2.4%, respectively. The median of sperm DNA fragmentation index (DFI) of those whose wives had a history of adverse pregnancy was 20.25% (95% CI [2.15-68.25]%). Multivariate logistic regression analysis suggested that mental stress (OR 1.567, 95% CI [1.081-2.27]) and sedentariness (OR 1.772, 95% CI [1.211-2.592]) were independent risk factors for asthenospermia.</p><p><b>CONCLUSION</b>The sperm quality of preconception males in Nanjing area is not encouraging, and it can be improved by changing undesirable lifestyle and reducing mental stress.</p>

Treatment of varicocele by microsurgical varicocelectomy: report of 94 cases / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the clinical effect of microsurgical varicocelectomy in the treatment of varicocele.</p><p><b>METHODS</b>We retrospectively analyzed the clinical data of 94 varicocele patients treated by microsurgical varicocelectomy, of whom 36 complained of testicular pain or dragging and distending discomfort, and 58 infertility or oligoasthenospermia. Microsurgical varicocelectomy was performed through inguinal or subinguinal approach. Complications were observed and semen parameters detected through follow-up visits at 1, 3 and 6 months after the operation.</p><p><b>RESULTS</b>Testicular pain or discomfort disappeared in 21 (65.6%) of the 32 followed-up patients who had complained of such symptoms, was relieved in 6 (18.8%), and remained unimproved in the other 5 (15.6%). The 56 followed-up patients with infertility or oligoasthenospermia all showed significantly improved sperm concentration and motility (grade a + b sperm), (15.47 +/- 3.21) x 10(6)/ml and (13.34 +/- 5.16)% at 3 months, and (18.39 +/- 4.05) x 10(6)/ml and (17.23 +/- 4.69)% at 6 months after operation, as compared with (8.26 +/- 1.68) x 10(6)/ml and (5.25 +/- 1.09)% preoperatively (P < 0.01).</p><p><b>CONCLUSION</b>Microsurgical varicocelectomy can effectively improve symptoms and semen parameters in varicocele patients.</p>