ABSTRACT
<p><b>OBJECTIVE</b>According to test results of the Hospital of AIDS screening laboratory in 2008, after counting analysis to assess the prevalence of AIDS, we can early detect positive cases in the future and effectively control the spread of AIDS.</p><p><b>METHODS</b>All serum samples were screened by ELISA method and we reexaminated the samples by PA. As long as one result is positive by the two methods, then we sent the positive samples to Beijing Center for Disease Control and Prevention by Western Blot method to confirm the result.</p><p><b>RESULTS</b>A total of 21 467 samples were detected and 29 (13.5% 0) were positive screening results. We confirm there were 7 (24.1%) positive samples and 12 (41.4%) suspected samples. We researched the epidemiology of the specimens by its source and age and sex.</p><p><b>CONCLUSION</b>Application of ELISA method for HIV screening test has a practical significance, it is accurate and fit to record the results of the screening test for AIDS.</p>
Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Infant , Male , Middle Aged , Young Adult , Acquired Immunodeficiency Syndrome , Blood , Diagnosis , Epidemiology , Age Distribution , China , Epidemiology , Enzyme-Linked Immunosorbent Assay , HIV Antibodies , Blood , Hospitals , Mass ScreeningABSTRACT
<p><b>OBJECTIVE</b>To study the in vitro antiviral effect of ribavirin combined with an oral preparation of traditional Chinese medicine "Hu Fei" (protecting the lung) on respiratory syncytial virus (RSV).</p><p><b>METHODS</b>Cytopathic effects (CEP) of RSV on Hep2 cells were observed after adding different concentrations of ribavirin, Hu Fei and combination of both into the culture medium.</p><p><b>RESULTS</b>The minimum concentration of ribavirin and Hu Fei for complete inhibition of CPE caused by RSV was 7.80 microg/ml and 5.00 mg/ml, respectively. When the combination of ribavirin and Hu Fei was applied, their minimum concentrations needed for complete inhibition were decreased to 0.98 ?g/ml and 0.63 mg/ml.</p><p><b>CONCLUSIONS</b>Both ribavirin and Hu Fei showed in vitro anti-RSV effect, but the inhibitory effect of combined ribavirin and Hu Fei was more potent than either of the preparation alone.</p>