Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons / 解剖学报

Objective To study the temporal and spatial expressions of G protein-coupled receptor, putative receptor protein related to angiotensin type 1 receptor (APJ), in mammal cochlea postnatal development. Methods The cochlear tissues of each group 11 C57BL/ 6 mice at postnatal day 7 (P7), P14, P28 and postnatal month 2(P2M) were taken out under a stereo microscope. Real-time PCR, Western blotting and immunofluorescent staining were used to detect the expressions of APJ in hair cells and spiral ganglion neurons. Results The expression pattern of APJ in cochleae showed an upward trend during the period from P7 to P2M. The temporal expressions of APJ in hair cells and spiral ganglion neurons increased obviously at P14 and P2M. The spatial expression patterns of APJ in hair cells and spiral ganglion neurons followed a declined gradient from base turns to apex turns at P14. Conclusion APJ expression exhibits a specific spatial and temporal pattern during mouse cochlea postnatal development, and may play a role in cochleae maturation and hearing formation.

In vitro anti-platelet aggregation effects of fourteen fruits and vegetables

In the present study, the anti-platelet aggregation activity of 14 vegetables and fruits was tested in vitro. The aqueous, 90% ethanol and ethyl acetate extracts, as well as concentrated juices of 14 foods [fruits and vegetables] were prepared, and the anti-platelet aggregation activity of those extracts was analyzed on a platelet aggregation analyzer in vitro with adenosine 5'-diphosphate [ADP], bovine thrombin [THR] and arachidonic acid [AA] as aggregation inducers, respectively. Aspirin [ASP] was used as the positive control. A number of the tested foods had inhibitory effects in concentration-dependent manner on platelet aggregations induced by various agonists. Especially, some foods such as lemon, leek, garlic, scallion, ginger, tomato and grapefruit showed good anti-platelet aggregation effect similar or higher than that of positive control group i.e. aspirin [ASP]. The results of present study provide scientific reference for reasonable selection of daily dietary with supplementary curative effects or prevention of cardiovascular diseases [CVD]

Anatomical study of the atypical azygos venous system and its clinical significance / 局解手术学杂志

Objective To investigate the morphology and classification of the atypical azygos venous system,and to provide reference for diagnosis and treatment of mediastinal and thoracic vascular diseases.Methods Thirty cadavers were perfused with 10%formalin solution, and immersed in the solution for one month before dissection.The vertebral levels of termination and diameters of the azygos,hemiazygos and accessory hemiazygos veins were examined.Results There were 24 cases of classical azygos venous system,which contained the azygos, hemiazygos and accessory hemiazygos veins.And there were 6 cases of atypical azygos venous system,which were further divided into 4 sub-groups.Namely,type a(2 cases)which showed an inverted"Y"shape;type b(2 cases)which was single column with hemiazygos and ac-cessory hemiazygos veins absent;type c(1 case)which showed an accessory hemiazygos vein draining into the left brachiocephalic vein;and type d(1 case)with azygos vein and double superior vena cava variation.Diameters of termination of the azygos,hemiazygos and accessory hemiazygos veins were(10.39 ±1.98)mm,(8.51 ±2.28)mm,and(6.29 ±1.56)mm,respectively.The vertebral levels of termination of the azygos,hemiazygos and accessory hemiazygos veins were T 4(83.3%),T7to T10,T4to T8respectively.Conclusion All morphometrical diameters and their termination levels can be used during preoperative CT evaluations before planning invasive mediastinal procedures.The re-sults of this study could be helpful in mediastinal surgery,mediastinoscopy and the surgery of the deformations of the vertebral column in the thoracic cavity.

Applications of platelets in studies on traditional Chinese medicines promoting blood circulation to remove blood stasis / 中国中药杂志

Thrombotic diseases in different forms become a great threat to human health. Such anti-platelet aggregation drugs as aspirin and clopidogrel are common drugs in clinic. However, along with the appearance of resistance and side effects of western anti-platelet aggregation drugs, anti-platelet aggregation traditional Chinese medicines promoting blood circulation to remove blood stasis have gradually become an important study orientation. Platelet is one of major participant in thrombosis, and plays an important role as a bioactive material in studies on traditional Chinese medicines promoting blood circulation to remove blood stasis, mainly involving two aspects--the evaluation for the anti-platelet aggregation activity of traditional Chinese medicines and the screening of their active components. This paper summarized the applications of platelets in studies on traditional Chinese medicines promoting blood circulation to remove blood stasis, so as to provide basis for further studies.

Effects of acupuncture on the sebum thickness in the female obese patient of different types / 中国针灸

<p><b>OBJECTIVE</b>To observe the influence of acupuncture on sebum thickness and the characteristic in the female obese patient of different types.</p><p><b>METHODS</b>Ninety-eight female obese patients were divided into 2 groups: an abdominal obesity group (n = 40) and an obesity group (n = 58). All of the patients in the two groups were treated by acupuncture at Zhongwan (CV 12), Chengman (ST 20), Tianshu (ST 25), Shuidao (ST 28), Qihai (CV 6), Zusanli (ST 36), Sanyinjiao (SP 6), etc. with electric stimulation, and in combination with ear point taping and pressing, for one month. Body weight index (BMI), waist circumference (WC), the sebum thickness of the upper limb (point A: at the inferior border of the right deltoid muscle and the midpoint of the lateral of arm), the trunk (point B: the inferior angle of right scapula) and the abdomen (point C: 3 cm right lateral to the navel; point D: the right anterior superior iliac) were observed and compared before and after treatment in the two groups.</p><p><b>RESULTS</b>There were significant differences before and after treatment in BMI, WC and the sebum thickness of points A, B, C and D in the two groups (P < 0.01), and the sebum thickness in the point C significantly changed as compared with those in other 3 points (P < 0.05) in the two groups. In the abdominal obesity group, change of BMI was very significantly positively correlated with changes of WC and sebum thickness in the point C, and significantly positively correlated to point A (P < 0.05), with the correlated coefficient [r(WC/BMI)] > [ r(C/BMI)] > [r(A/BMI); change of WC was very significantly positively correlated to point C (P < 0.01). In the obesity group, the change of BMI was very significantly positively correlated to WC (P < 0.01) and significantly positively correlated to the sebum thickness's change of point B (P < 0.05), while WC change was very significantly positively correlated to point B and C (P < 0.01) and significantly positively to point D (P < 0.05).</p><p><b>CONCLUSION</b>Acupuncture can effectively reduce the body weight, WC and subcutaneous fat in various parts of the body and selectively reduce the over fat of different places in both the obesity patient and the abdominal fat patient with exceeding standard of WC.</p>

Comparison of therapeutic effects between body acupuncture and scalp acupuncture combined with body acupuncture on atherosclerotic cerebral infarction at acute stage / 中国针灸

<p><b>OBJECTIVE</b>To compare therapeutic effects between body acupuncture and scalp acupuncture combined with body acupuncture on atherosclerotic cerebral infarction at acute stage.</p><p><b>METHODS</b>Forty-two cases of atherosclerotic cerebral infarction at acute stage were divided into 2 groups. They were treated respectively by scalp acupuncture combined with body acupuncture and simple body acupuncture. The comprehensive scores of mind, speech, limb motor function, etc. of the patients before and after treatment were recorded and compared.</p><p><b>RESULTS</b>After treatment, the cumulative scores in the two groups reduced significantly (P < 0.01); the difference of scores before and after treatment in the scalp-body acupuncture group was significantly higher than that in the body acupuncture group (P < 0.05); there was no significant difference between the two groups in clinically effective rate (P > 0.05), but there was a very significantly difference between the two groups in the markedly effective rate (P < 0.01), the scalp-body acupuncture group being significantly higher than that in the body acupuncture group.</p><p><b>CONCLUSION</b>Acupuncture can improve mind, speech and limb motor function of the patient with atherosclerotic cerebral infarction at acute stage, and the therapeutic effect of scalp combined with body acupuncture is better than that of simple body acupuncture.</p>

Observation on clinical therapeutic effect of scalp acupuncture combined with body acupuncture on apoplectic hemiplegia / 中国针灸

<p><b>OBJECTIVE</b>To search for the best method for increasing clinical therapeutic effect on apoplectic hemiplegia.</p><p><b>METHODS</b>One hundred and twenty cases of apoplectic hemiplegia were randomly divided into three groups in order of visiting. Group A (n = 33) were treated by scalp acupuncture combined with consciousness-restoring resuscitation method, group B (n = 60) by scalp acupuncture combined with traditional acupuncture, and group C (n = 27) by traditional acupuncture. The clinical therapeutic effects were evaluated according to the scores of manner, speech, motor function of limbs, and so on.</p><p><b>RESULTS</b>Three cases were basically cured, 20 were markedly effective, 7 were effective, with a total effective rate of 90.9% in the group A; and the corresponding figures were 7, 28, 21 cases and 93.3%, respectively in group B with no significant difference between the group A and B (P > 0.05); and 7 cases were markedly effective, 11 effective with a total effective rate of 66. 7% in the group C with a significant difference as compared with the group A and B (P < 0.05, P < 0.001).</p><p><b>CONCLUSION</b>Scalp acupuncture combined with consciousness-restoring resuscitation method and scalp acupuncture combined with traditional acupuncture have a similar therapeutic effect on apoplectic hemiplegia, which is superior to that of traditional acupuncture.</p>

Effects of acupuncture on waist circumference and body mass index in females of obesity of different types / 中国针灸

<p><b>OBJECTIVE</b>To observe slimming effect of acupuncture for females of obesity of different types, and search for the best indication of acupuncture.</p><p><b>METHODS</b>Eighty-three cases were divided into two groups: an abdominal obesity group (n = 31) with exceeding standard waist circumference (WC) and Body mass index (BMI)< 25, and an obesity group (n = 52) of simple obesity (BMI>25). They were treated by same acupuncture therapy for one month.</p><p><b>RESULTS</b>BMI, WC and the ratio of WC to hip circumference (WHR) lowered in the two groups with very significant differences as compared with those before treatment (P<0.01 or P<0.05). In the abdominal obesity group, changes of WC and WHR were positively correlated to changes of BMI (P<0.01 or P<0.05). In the obesity group, WC was positively correlated to BMI (P<0.01) and WHR was not significantly correlated to BMI (P>0.05).</p><p><b>CONCLUSION</b>Acupuncture has very good effects of slimming and reducing the waist circumference for the female of abdominal obesity with exceeding standard WC, or the patient of simple obesity, and it is suitable to obesity of different types.</p>