ABSTRACT
Objective To evaluate the efficacy and safety of continuous femoral nerve block (CFNB) versus patient controlled analgesia (PCA) for analgesia in primary total knee arthroplasty (TKA) through a Meta-analysis.Methods All randomized controlled trials (RCTs) which compared about the efficacy and safety of CFNB versus PCA for analgesia in primary TKA,were searched from Cochrane Library,Embase,PubMed,CBM,VIP,Wang Fang database and CNKI.At the same time,conference papers were identified manually.A quality assessment of the included literature was evaluated by Cochrane system evaluation manually.Revman 5.3 software was used for the Meta-analysis to compare visual analogue scale (VAS),hospital for special surgery knee score (HSS),western Ontario and McMaster university of orthopedic index (WOMAC),patient satisfactory degree,and incidences of complications (nausea,vomit,dizziness,somnolence,itch of skin,etc).Results Twenty-four RCTs involving 1896 patients were identified including CFNB (946 cases) and PCA (950 cases) application of TKA.The pooled results illustrated that CFNB could reduce VAS in rest (MD =-1.28,95％ CI-1.56,-1.00,P ＜ 0.05) and VAS in movement (MD =-0.98,95％ CI-1.38,-0.58,P ＜ 0.05),increase HSS (MD =2.13,95％ CI 0.12,4.14,P ＜0.05),reduce WOMAC(MD =-0.97,95％ CI-1.83,-0.11,P ＜ 0.05),increase patient satisfactory degree (RR =1.27,95 ％ CI 1.10,1.46,P ＜ 0.05) and at the same time reduce the incidences of nausea,vomit,dizziness,somnolence and itch of skin (RR =0.32,95％CI0.24,0.43,P＜0.05).Conclusion Compared with PCA,CFNB analgesia can reduce VAS,WOMAC score,improve the postoperative HSS and patient satisfaction degree,and decrease the risk of complications.
ABSTRACT
Objective To evaluate the efficacy and safety of continuous femoral nerve block (CFNB) versus patient controlled analgesia (PCA) for analgesia in primary total knee arthroplasty (TKA) through a Meta-analysis.Methods All randomized controlled trials (RCTs) which compared about the efficacy and safety of CFNB versus PCA for analgesia in primary TKA,were searched from Cochrane Library,Embase,PubMed,CBM,VIP,Wang Fang database and CNKI.At the same time,conference papers were identified manually.A quality assessment of the included literature was evaluated by Cochrane system evaluation manually.Revman 5.3 software was used for the Meta-analysis to compare visual analogue scale (VAS),hospital for special surgery knee score (HSS),western Ontario and McMaster university of orthopedic index (WOMAC),patient satisfactory degree,and incidences of complications (nausea,vomit,dizziness,somnolence,itch of skin,etc).Results Twenty-four RCTs involving 1896 patients were identified including CFNB (946 cases) and PCA (950 cases) application of TKA.The pooled results illustrated that CFNB could reduce VAS in rest (MD =-1.28,95％ CI-1.56,-1.00,P ＜ 0.05) and VAS in movement (MD =-0.98,95％ CI-1.38,-0.58,P ＜ 0.05),increase HSS (MD =2.13,95％ CI 0.12,4.14,P ＜0.05),reduce WOMAC(MD =-0.97,95％ CI-1.83,-0.11,P ＜ 0.05),increase patient satisfactory degree (RR =1.27,95 ％ CI 1.10,1.46,P ＜ 0.05) and at the same time reduce the incidences of nausea,vomit,dizziness,somnolence and itch of skin (RR =0.32,95％CI0.24,0.43,P＜0.05).Conclusion Compared with PCA,CFNB analgesia can reduce VAS,WOMAC score,improve the postoperative HSS and patient satisfaction degree,and decrease the risk of complications.
ABSTRACT
Objective To explore the angiogenesis&neovascularization effects of naringin treatment in ovariectomized rats’fracture healing. Methods Upper 1/3 transverse tibial fracture model 4 weeks later after ovariectomized were estimated and randomly divided into the naringin group and control group. Microfil perfusion technique was used to analysis the angiogene?sis situation at two weeks after bone fracture. HE staining was used to evaluate the level of angiogenesis&neovascularization of tis?sue from histological point of view. The relative expression of VEGF in the callus was identified by real?time polymerase chain re?action. Immunohistochemical technique was used to observe the vessel endothelial growth factor?2 in the callus of the two groups. Maximum fracture load was tested by three?point bend test. Results The vascular volume and vascular density were more in nar?ingin group than control group. The HE staining of the 2 week group slices shows that the VA, VN2 of the unit of high magnifica?tion vision of the naringin group was significantly larger compared to the control group. Real?time PCR revealed that the compara?tive expression of VEGF is more in naringin group than in control group; the positive number of VEGFR?2 is more in naringin group than in control group. Naringin can promote the maximum load of the callus. Conclusion Naringin can promote ovariecto?mized rats’angiogenesis&neovascularization in the early process of fracture healing. It may be act on the signaling pathway of VEGF/VEGFR?2.
ABSTRACT
Alignment-free comparison is a recently developed method for sequence alignment, which has high computational efficiency and suitable to the low identical sequences. Alignment-free comparison was successfully applied in the DNA analysis. However, the accuracy of analysis is not high when it was applied in protein analysis because the complexity of protein is larger than DNA by consisting of 20 types of residues. Thus, residues are clustered into a few groups based on their similarity of physicochemical features. Using such simplified alphabets, the complexity of protein sequences is reduced and at the same time the key information encoded in the sequences remains. Therefore, the accuracy of alignment-free comparison is improved.