ABSTRACT
Objective:To investigate the suppressed e ects of total hedysarum polybotrys saccharide(THPS) alone and THPS combined with Cytoxan(CTX) on tumor-bearing mice and its mechanisms.Method:Kunming mice inoculated with S180 were given low,moderate and high dose THPS and THPS combined with CTX by intraperitoneal injection.Fourteen day later,tumor weight,inhibition rate,thymus index and spleen index were observed.CD4+ and CD8+ lymphocyte were counted though the ow cytometer.Results:Compared with the control,the moderate dose THPS showed signi cantly suppressed e ect on the growth of S180 tumor(P
ABSTRACT
AIM: To study effects and mechanisms of total hedysari polysaccharide(THPS)inducing apoptosis of S180 tumor. METHODS: 60 Kunming mice were divided into 5 groups randomly after inoculating S180 tumor.Sample group(S) and cyclophosphamide group(CY) were injected intraperitoneally with normal sodium and cyclophosphamide respectively.Low dose THPS group(H100),moderate dose THPS group(H200),and high dose THPS group(H400) were injected intraperitoneally with low,moderate,and high dose THPS respectively.The mice were killed after being treated for 14 days.Appearance of tumor was observed by electron microscope,and DNA,RNA,intracellular (Ca~(2+)),free radicals,mitochondrial membrane potential of tumor were determined by laser scanning confocal microscope. RESULTS: Moderate dose THPS raised intracellular Ca~(2+),free radicals and reduced mitochondrial membrane potential and DNA,RNA of tumor. CONCLUSION: Moderate dose THPS induces apoptosis of S180 tumor through raising intracellular Ca~(2+),free radicals and reducing mitochondrial membrane potential.