ABSTRACT
Objective To study the influence of serum containing Ziqi Ruangan Pill on inhibitory effect of alcohol LO2 and to investigate the regulation on apoptosis related genes and the protective mechanism for LO2. Methods Rabbit sera saline containing Ziqi Ruangan Pill was prepared. The apoptotic LO2 model was established by being cultured with alcohol. The effect of serum on the model was detected by MTT method. Identification of apoptosis was carried out using flow cytometry and Hoechst3325 fluorescent staining. The expression of apoptotic inducing gene,casepase3 and NF-?B was detected by Real-time RT PCR and Western-Blot. Results The serum containing Ziqi Ruangan Pill could regulate the expression of the casepase3,NF-?B and reduce the apoptosis. Conclusion Ziqi Ruangan Pill can protect the LO2 by regulating the expression of apoptosis stimulative gene casepase3 and NF-?B.
ABSTRACT
Objective To study protective effects of vitamin E(VE) on renal proximal tubular cells of mice treated with cadmium chronically. Methods 75 Kunming mice were divided into 3 groups randomly, cadmium group, VE group, control group. The mice in cadmium group were treated with cadmium in 2 mg/kg by subcutaneously injected, twice per week, in VE group they were treated with VE in 10 mg/(kg?d) additionally, in control group the animals were treated with saline only. 3 months later the finestuctural changes of the renal proximal tubule cells were observed by electron microscopy and immunohistochemistry(Tunel method). The ultrastuctures of nuclei were revealed with stereological analysis. The apoptotic cells were counted with image analysis. Results The structure of the renal proximal tubule cells of mice in the VE group was similar to those in the control group, but it had significant changed compared with those of the cadmium group. Compared with the control group, the nuclear important morphological parameters of VE group increased significantly (P
ABSTRACT
Thyroid glands from 80 human fetuses, 7～31 weeks in gestational age were obtained to study the morphological development of thyroid glands by histochemical, immunohistochemical and TEM techniques. During 7～8.5 weeks the glands composed mostly of epithelial cell cords and abundant glycogen were seen in the cytoplasm of the cells. Some follicles and small accumulations of colloid in the center of the follicles appeared at 12～12.5 weeks and less glycogen was found in the cells. C cells were distributed mainly in the posterior region of the upper pole of both lobes, they usaually occurred singly or in small groups. During intrauterine life, C cells occupied three positions. They are found between follicular cells, between follicular cell and basement membrane and between follicles. But they are situated predominantly in the follicular wall. Three morphological variants were found in the human fetal C cells: (1) spherical or oval cells, (2) polygonal or pyramidal cells (3) cells with a cytoplasmic process. Developing C cells seem to have few secretory granules by TEM and argyrophilic reaction in semithin sections.