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Purpose@#Oxidized cellulose is available in many forms, but manufactured using either a regenerated or non-regenerated process. In this study, we evaluated the effects of 2 different hemostatic agents for the treatment of local bleeding in patients undergoing hepatic resection. @*Methods@#This was a monocentric, parallel-group, randomized, and controlled clinical trial to compare oxidized regenerated cellulose gauze (ORCG) with oxidized non-regenerated cellulose gauze (ONRCG) in patients undergoing hepatectomy. The primary endpoint was the time to hemostasis at the target bleeding site. The secondary endpoints were the postoperative drainage volume on the first 2 days after surgery and the hospital stay. @*Results@#There was no significant difference between the ORCG and ONRCG groups in time to hemostasis from column analysis (238.8 ± 121.6 seconds vs. 193.7 ± 85.3 seconds, P = 0.068), and there were no differences in the rates of hemostatic success between the 2 groups at 120 seconds (18.4% vs. 24.3%; odds ratio [OR], 0.703; 95% confidence interval [CI], 0.231–2.136) and 300 seconds (71.1% vs. 89.2%; OR, 0.298; 95% CI, 0.085–1.041). However, the ONRCG group was superior to the ORCG group in hemostasis according to the survival analysis (log-rank test, P = 0.044). Moreover, there were also no significant differences between the 2 groups in postoperative drainage volume on the first 2 days (P = 0.436, P = 0.381) and hospital stay (P = 0.537, P = 0.200). @*Conclusion@#ONRCG was not inferior to ORCG as a hemostatic agent in patients undergoing liver resection.
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Purpose@#Oxidized cellulose is available in many forms, but manufactured using either a regenerated or non-regenerated process. In this study, we evaluated the effects of 2 different hemostatic agents for the treatment of local bleeding in patients undergoing hepatic resection. @*Methods@#This was a monocentric, parallel-group, randomized, and controlled clinical trial to compare oxidized regenerated cellulose gauze (ORCG) with oxidized non-regenerated cellulose gauze (ONRCG) in patients undergoing hepatectomy. The primary endpoint was the time to hemostasis at the target bleeding site. The secondary endpoints were the postoperative drainage volume on the first 2 days after surgery and the hospital stay. @*Results@#There was no significant difference between the ORCG and ONRCG groups in time to hemostasis from column analysis (238.8 ± 121.6 seconds vs. 193.7 ± 85.3 seconds, P = 0.068), and there were no differences in the rates of hemostatic success between the 2 groups at 120 seconds (18.4% vs. 24.3%; odds ratio [OR], 0.703; 95% confidence interval [CI], 0.231–2.136) and 300 seconds (71.1% vs. 89.2%; OR, 0.298; 95% CI, 0.085–1.041). However, the ONRCG group was superior to the ORCG group in hemostasis according to the survival analysis (log-rank test, P = 0.044). Moreover, there were also no significant differences between the 2 groups in postoperative drainage volume on the first 2 days (P = 0.436, P = 0.381) and hospital stay (P = 0.537, P = 0.200). @*Conclusion@#ONRCG was not inferior to ORCG as a hemostatic agent in patients undergoing liver resection.
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Objective@#To explore the effects of workplace health promotion in a wind power equipment manufacturing factory.@*Methods@#Based on investigation of occupational hazard factors, personal protective equipment (PPE) application, occupational health management and healthy life style, health promotion strategy and intervention were implemented in this factory. The sample size was 56 for monitoring of occupational hazard factors before and after intervention, 283 and 259 for questionnaire before and after intervention.@*Results@#After intervention, the qualified rate of workplace occupational hazard factors increased from 67.9% to 82.1%. The abnormal rate of occupational health surveillance among workers decreased from 24.7% to 11.6%. The rates of correct use of PPE increased from 39.6% to 80.7%. The rates of awareness of occupational health increased from 10.2% to 71.8%. The rates of awareness of chronic disease increased from 10.2% to 77.2%. Two-week consultation rate decreased from 6.4% to 4.2%. Smoking rate decreased from 12.4% to 10.4%.@*Conclusion@#Workplace health promotion is effective measures for reducing occupational hazard factors exposure and improving workers’ health.
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By searching and analizing the related literatures of recent years, the extraction methods, biological activites and application of yeastβ-glucan were reviewed.It is found that there are many kinds of extraction methods, including acid method, alkali method, acid-alkali method, alkali-enzymatic method, autolysis-enzyme-alkali method, induced autolysis-alkali method, microwave assisted alkali-enzyme method, autolysis-ultrasonic coupling method, enzymatic method, etc.There are lots of biological activity study report on yeast β-glucan, envolving the activities of immune, anti-tumor, anti-oxidant, anti-radiation, lowering blood lipid, and regulating intestinal flora as prebiotics, etc.Yeast β-glucan is widely used in animal breeding industry, food industry and cosmetics industry.From the review we can know that seeking efficient, fast, pollution-free yeastβ-glucan extraction methods is the hot research point, and it will have more applications in food and cosmetics industries as good biological response regulator in the future .
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Objective To construct a eukaryotic expression vector in Pichia pastoris containing human fibrinogen gene, in order to achieve high level secretory expression in extracellular.Methods Expression plasmid,pGAPZαA-FGB-FGG-FGA-AOX1,was constructed by inserting the synthesized sequence encoding human fibrinogen(FGA, FGB,FGG) and then introduced into Pichia pastoris SMD1168H by electroporation.Transformants were availably screened by Zeocin resistance,the expression of recombinant protein was identified by SDS-PAGE and Western blot analysis, the protein yield was tested by ELISA assay.After ultrafiltration and purification, the biological activity of protein was detected.Results The crude yield of human fibrinogen in Pichia pastoris supernatant reached 15 mg/L in flask and the biological aggregation activity was determined.Conclusion The human fibrinogen gene was obtained and successfully expressed in Pichia pastoris and the active products were secreted into the medium.
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Objective To explore the influence of abnormal donor hepatic artery on hepatic artery and biliary complications after liver transplanta?tion,and summarize the hepatic artery reconstruction procedures during transplantation. Methods The clinical data of 210 cases of liver transplan?tation conducted in our hospital from May 2005 to April 2015 were retrospectively searched for the study,including 42 with abnormal donor hepatic artery. Results Among the 210 liver transplantation,42 cases exhibited abnormal donor hepatic artery,and the aberration rate was 20.0%. Mean volume of blood flow of abnormal group and normal group was 4.7±95.1 mL/min and 190.9±101.6 mL/min,respectively. There was no statistic differ?ence(P=0.519). Twelve cases had arterial complications,the incidence rate was 5.71%,and there was no statistic difference between each group (χ2=0.72,P>0.05). Twenty five cases got biliary complications,the incidence rate was 11.9%,and there was no statistic differences between each group(χ2=0.05,P>0.05). Conclusion There was no statistic difference of mean volume of blood flow after arterial reconstruction between two groups. Liver transplantation with abnormal arterial reconstruction will not increase the incidence rate of arterial and biliary complications.
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Objective To study effect of Taxol on protein O-GlcNAcylation levels and investigate whether protein O-GlcNAcylation levels can affect the sensitivity of breast cancer cells to Taxol.Methods Western blot analysis was performed to examine protein O-GlcNAcylation levels and the expression of enzymes related to O-GlcNAcylation biosynthesis in Taxol treated breast cancer cells.RT-qPCR was used to analyze the effects of Taxol on OGA and OGT mRNA expression in cancer cells.The sulforhodamine B colorimetric assay was used to determine the effect of alteration of protein O-GlcNAcylation on the anti-proliferation of Taxol in breast cancer cells by adding OGA inhibitor and OGT inhibitor, respectively.Results Taxol treatment enhanced protein O-GlcNAcylation levels in dose-and time-dependent manners in breast cancer cell MDA-MB-231 ( P <0.05 ) .Taxol increased the mRNA levels of OGT and OGA after MDA-MB-231 cells were treated for 24 h(P<0.05).As OGA inhibitors increased protein O-GlcNAcylation levels, the sensitivity of MDA-MB-231 to Taxol was increased.As OGT inhibitor decreased protein O-GlcNAcylation levels, the sensitivity of MDA-MB-231 to Taxol was reduced.Conclusion Taxol treatment can enhance protein O-GlcNAcylation levels and the changes of O-GlcNAcylation levels alter the sensitivity of breast cancer cell MDA-MB-231 to Taxol.
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Chondroitin sulfate proteoglycans ( CSPGs) is one kind of proteins that covalently bind with chondroitin sulfate.CSPGs play important roles in the growth and development of the central nervous system and the pathological reaction of nervous injury.This article reviews the functional and mechanism studies of CSPGs in the repair of nerve system injury.
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Intestinal microbiota is closely related to the human health. The unhealthy state is often associated with disorders in intestinal microbiota. Intestinal microbiota modulators such as probiotics, prebiotics and synbiotics, which can restore and improve intestinal microbiota balance, are thus drawing wide attention. This paper reviewed the research progress of modulators of intestinal microbiota.
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Objiective To prepare pingyangmycin water-soluble wax stick and to establish its content determination method. Method The formula was optimized by orthogonal experiment and the content of pingyangmycin in wax stick was determined by high performance liquid chromatography (HPLC). Results The formula of pingyangmycin wax stick matrix was optimized as 1 g of alcohol ethoxylate and 1 g of S 40. Pingyangmycin was added when the temperature of the matrix raised to 70℃and stirred for 20 min. The linear range of pingyangmycin determined by HPLC was 34.4~172μg/mL,the regression equations was y=8298.9 x-34996(r=0.9999),and the average recovery of pingyangmycin was 102.67%(n=9). Conclusion The Preparing procedure of pingyangmycin water-soluble wax sticks is simple and stable. The HPLC method for determining the content of pingyangmycin in water-soluble wax sticks is simple, fast and accurate.
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Objective To summarize the clinical experience of harvesting and using the kidneys from donation after cardiac death (DCD) donors.Methods Fourteen kidney transplantations were successfully performed on 14 patients with end-stage renal diseases.The kidneys were harvested from 7 volunteer donors (age 30～53 years) diagnosed with cardiac death,who were scored 19～23according to the University of Wisconsin donation after cardiac death evaluation.Primary diseases of the donors were cerebral hemorrhage,brain injury,ischemic cerebral vascular disease and brain tumor.Warm ischemia time ranged from 5 to 45 min,and cold ischemia time was 4.5 ～ 12.5 h.Results After transplantation,three patients had delayed graft function (DGF),one had primary non-function (PNF),and two patients developed acute rejection.In the patient with PNF,the transplanted kidney was removed one day after operation and the patient went back to hemodialysis.One patient with DGF was still in recovery with serum creatine 149 μmnol/L (within 3 months after operation).The above two cases both utilized the kidneys with 45 min of warm ischemia time.The rest 12 patients were discharged with normal renal function.Conclusion Under the condition of our country,kidneys strictly harvested from DCD donors can be used as one of the main sources of kidney grafts for kidney transplantation.
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Objective To summarize our experience in the liver transplantation from the donation after cardiac death (DCD).Methods The livers from three DCD donors (2 cases of brain trauma and 1 case of cerebral hemorrhage) were harvested according to the Guidelines for Donation after Cardiac Death in China.These grafts were orthotopically transplanted into three recipients including 2 cases of decompensative hepatic cirrhosis and 1 case of primary liver cancer.The warm ischemic time ranged from 7.5 to 10 min and the cold ischemic time was 4.5,8.2 and 6.5 h respectively.Postoperative immunosuppressive regimens included prednisone,FK506 and mycophenolate mofetil (MMF).Antibiotics and anticoagulatants were used accordingly.Results All of the 3 recipients obtained normal liver function within 3 weeks since the grafts were implanted without PNF,thrombosis and rejection.No postoperative complications occurred in 3 recipients during the follow-up period of 2 to 9 months with normal liver function.Conclusion The liver transplant from DCD donor showed good results in our center.Chinese group Ⅲ of DCD donor,UW score above the middle level and the short warm ischemic time are three keys ensuring the success of the liver transplant from DCD donors.
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Objective To investigate the risk factors associated with acute renal failure (ARF)after surgery in patients with simple abdominal trauma. Methods A retrospective case-control study was carried out in patients with simple abdominal trauma after surgery from January 2003 through May 2008 in our hospital. Twenty patients with ARF were set as study group and 68 patients without ARF as control group. Clinical data including age, gender, injury type, number of injured organs, blood transfusion volume, intra-abdominal pressure, abdominal infection, shock and related factors were analyzed by using univariate and Logistic regression to identify the independent risk factors of ARF after surgery in patients with simple abdominal trauma. Results Univariate analysis revealed statistical difference in aspects of blood transfusion volume ( ＞ 1 600 ml), operation timing ( interval between trauma and operation ＞12 h), preoperative shock duration ( ＞6 h), postoperative abdominal infection and intra-abdominal hypertension ( ＞ 12 mm Hg) between two groups ( P ＜ 0. 05 ). Logistic regression analysis showed that these five indices were the independent risk factors of ARF after surgery in patients with simple abdominal trauma ( P ＜ 0. 05 ). Conclusions Massive blood transfusion, delayed operation, long preoperative duration of shock, postoperative abdominal infection or intra-abdominal hypertension are the risk factors of ARF after surgery in patients with simple abdominal trauma. We should pay attention to these factors and take effective measures to prevent occurrence of ARF.
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Objective To detect the expression of KL-6 mucin in the tissue and serum of hepatoma in different hepatoma patients,and to investigate the value of KL-6 mucin as a tumor marker in the diagnosis of hepatoma.Methods The expression of KL-6 mucin in the hepatoma tissues of 81 patients with hepatocellular carcinoma(HCC),21 patients with cholangiocarcinoma(CC),12 patients with combined hepatocellular and cholangiocarcinoma(HCC-CC)and 56 patients with metastatic liver cancer(MLC)was detected by immunohistochemical analysis.The expression of KL-6 mucin in the serums of 34 HCC patients,8 CC patients,30 MLC patients and 19 healthy individuals was detected by enzyme-linked immunosorbent assay,and all the data were analyzed by t test.Results Expression of KL-6 mucin was detected in the cholangiocarcinoma tissues in all CCand HCC-CC patients.In several hepatoma cells and partial hepatoma tissues of patients with MLC,the expression of KL-6 mucin was detected.No expression of KL-6 mucin was detected in the hepatocellular carcinoma tissuesand non-cancerous tissues of patients with HCC or HCC-CC.The serum levels of KL-6 mucin expression in CC patients were signifcantly higher than those in healthy individuals,HCC and MLC patients(t=5.58,5.34,4.00.P＜0.01).The difference of the serum levels of KL-6 mucin expression between MLC patients and healthy individuals had statistical significance(t=2.77,P＜0.01).However,no significant difference in serum levels of KL-6 mucin expression was found between HCC patients and healthy individuals and between HCC patients and MLC patients(t=2.03,1.89,P＞0.05).Conclusion The expression of KL-6 inucin in CC patients is significantly higher than in patients with other types of hepatoma in both tissue and serum levels.Thus,KL-6 may be a usetul new tmnor marker for distinguishing CC from other types of hepatoma.
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BACKGROUND:When immunological rejection occurs following liver transplantation,liver cells are destroyed by infiltrated T lymphocytes,leading to progressive deterioration of hepatic function owing to reduction of liver cells.Induction of immunological tolerance of liver transplantation remains a challenge.OBJECTIVE:To observe the influences of pretransplant intraportal infusion of recipient blood into donor on the apoptosis of hepatic allograft-infiltrating T lymphocytes in rats.DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Organ Transplant Unit,China University between May 2002 and May 2004.MATERIALS:Inbred rats were developed into models of orthotopic liver transplantation.Twenty-four female ACI rats(RT1a)served as donors,and an additional twenty-four male LEW rats(RT11)served as recipients.METHODS:A modification of cuff method was employed for orthotopic liver transplantation in rats.Twenty-four recipient rats recipient blood was infused into each donor rat via the portal vein.All blood infusions were performed 7 days prior to liver transplantation.MAIN OUTCOME MEASURES:Rat survival time,serum content of γ- interferon,histological changes of hepatic allograft,number of dendritic cells in the hepatic allograft,and T lymphocyte apoptosis following liver transplantation.RESULTS:Rat survival time was significantly longer in the intraportal infusion of non-recipient blood group than in the control group(P＜0.01).At 3 and 5 days after liver transplantation,the intraportal infusion of non-recipient blood group exhibited significantly higher serum content of y- interferon than the control group(P＜0.05).No significant differences in rat survival time and serum content of γ- interferon were found between intraportal infusion of non-recipient blood and intraportal infusion of non-recipient blood groups and control group(P＞0.05).In the intraportal infusion of non-recipient blood group,infiltrated T lymphocytes in the hepatic allograft were significantly reduced,and a large number of donor-sourced dendritic cells were detected.The number of apoptotic cells per square millimeter of hepatic tissue was significantly higher in the intraportal infusion of non-recipient blood group than in the control group(P＜0.01).CONCLUSION:Pretransplant intraportal infusion of recipient blood into donor can prolong the survival time of hepatic allograft and promote the apoptosis of hepatic allograft-infiltrating T lymphocytes.
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Objective To evaluate intraoporative ultrasonography (IOUS) of liver tumors. Methods In this study, 45 patients with liver tumors were examined by the IOUS(Logiq 500 scanner with a dedicated 5 ～ 7.5 MHz linear-array intraoperative transducer), and the results of IOUS were compared with those of preoperative ultrasonography (PHILIPS HDI 5000 scanner with a 3.5 ～ 5 MHz convex-array transducer) and computed tomography (CT). Results For tumors with a diameter ≥ 1 cm, the detection rate of IOUS was 100%, which was higher than that of preoperative ultrasonography and CT (100% vs 89.80% and 97.96%), though there was no statistical significance(xc2 = 3.372 ,P >0.05 and Fisher exact probability was 1.00) ; For tumors with a diameter < 1 cm, IOUS had a significantly higher detection rate than preoperative uhrasonography and CT(90.70% vs 62.79% and 74.42%, respectively,the former x2= 9.382, the latter x2 = 3.957, both P < 0.05) ;The total detection rate of IOUS was significantly higher than that of preoperative ultrasonography and CT (95.65% vs 77.17% and 86. 96%, respectively, the former x2 = 13.378, the latter x2 = 4.381, both P < 0.05). All the detected lesions underwent surgical resection or intratumoral injection of ethanol with the guidance of IOUS. Conclusions Compared with preoperative imaging examination, IOUS not only can enhance the detection rates of preoperatively overlooked lesions, but also can provide more valuable information to guide the surgical procedures.
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Aim To investigate the protective effects of LMWH-SOD(Low molecular weight heparin- Superoxide dismutase Conjugate)on the injuries induced by oxygen and glucose deprivation in cultured neurons. Methods The cortical neurons of fetal rat were cultured in vitro. The antioxidant and protective effects of LMWH-SOD were observed by treating neurons with oxygen and glucose deprivation. Results LMWH-SOD reduced the number of cell death and the efflux of LDH and the content of NO,MDA and increased the membrane fluidity after the injuries of cells. Conclusion LMWH-SOD has protective effects on cerebral cortical neurons through its action of scavenging free radicals.
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One kind of glycosaminoglycans (GAG) was extracted and purified from the ink of Sepiella maindroni de Rochebrune by using the procedures of trypsin and pronase digestion, ethanol precipitation and DEAE-cellulose inon-exchange chromatography. It was proved to be homogeneous by polyacrylamide gel and cellulose acetate membrane electrophoresis. No protein absorption was shown in UV spectrum, but typical absorption of GAG was shown in the IR spectrum. It was identified by paper and thin layer chromatography of the acid hydrolyzate that the GAG was composed of galactosamine, glucuronic acid and fucose. The percentage content of galactosamine, glucuronic acid, fucose, sulfate and protein in the GAG was 25. 6%, 29.2%, 23.3%, 8.2% and 3. 5%, respectively.
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DNA extraction is a basic technology of molecular biology. The purity and the integrality of DNA structure are necessary for different experiments of gene engineering. In recent years there have been some new or improved DNA extraction methods appeared. The methods of DNA extraction from animals, plants, microorganisms and marine organisms were summarized in this article.
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Objective To evaluate the diagnostic method for and surgical effect on hilar cholangiocarcinoma. ] Methods The clinical features, diagnosis, surgical therapy and long term result were retrospectively analysed on 192 cases of hilar cholangiocarcinoma admitted into our hospital from Jan.1984 to Jun.1999Results] MRCP delineated 100%(44/44) of the hilar tumors. 153 cases underwent exploration. 88 cases treated by internal or external drainage have survived for 3～15 months. Tumor resection was performed on 53 cases, and orthotopic liver transplantation on 2 cases, with a resection rate of 36%(55/153) including radical resection in 38 cases (median survival period was 31 months). 17 cases undergoing palliative resection have a median survival period of 13 months. Conclusion MRCP was an ideal noninvasive preoperative examination for identifying hilar cholangiocarcinoma. Routine frozen section of the bile duct margin should be adopted. Radical resection definitely prolongs survival period. For Bismuth type Ⅲ、Ⅳ without extrahepatic metastasis, orthotopic liver transplantation was an effective surgical therapy.