ABSTRACT
Objective To investigate the clinical value of perinuclear antineutrophil cytoplasmic antibody (p-ANCA) and fecal calprotectin in predicting the severity of ulcerative colitis (UC) in children.Methods One hundred children with UC from March 2014 to March 2019 in Affiliated Dongfeng Hospital,Hubei University of Medicine were selected.According to the endoscopic severity index of ulcerative colitis (UCEIS),the children were divided into remission stage (29 cases);active stage (71cases),among whom 43 cases were mild-moderate,and 28 cases were severe.The serum levels of interleukin-6 (IL-6),procalcitonin (PCT),C-reactive protein (CRP),erythrocyte sedimentation rate (ESR),albumin,platelet,hemoglobin,white blood cell and p-ANCA were detected,and the fecal calprotectin was detected.The clinical value of each index in predicting the severity of UC was determined by receiver operating characteristic (ROC) curve.Results The course of disease,IL-6,PCT,CRP,ESR,p-ANCA and calprotectin in remission stage children were significantly lower than those in active stage children:(3.14 ± 1.25) years vs.(3.73 ± 0.89) years,(10.08 ± 4.40)/μg/L vs.(15.84 ± 3.22) μg/L,(1.02 ± 0.38) μg/Lvs.(1.38 ± 0.43) μg/L,(15.92 ± 6.13) mg/L vs.(24.30 ± 6.06) mg/L,(14.75 ± 6.42) mm/1 h vs.(25.31 ± 6.98) mm/1 h,(17.19 ± 4.76) U vs.(28.01 ± 6.12) U and (504.82 ± 127.46) μg/g vs.(717.04 ± 142.30) μg/g,and there were statistical differences (P＜0.05 or ＜0.01).The IL-6,CRP,ESR,white blood cell,p-ANCA and calprotectin in mild-moderate children were significantly lower than those in sever children:(14.56 ± 2.72) μg/L vs.(17.82 ± 2.93) μg/L,(22.01 ± 5.32) mg/L vs.(27.83 ± 5.46) mg/L,(22.31 ± 4.46) mm/1 h vs.(29.91 ± 7.70) mm/1 h,(7.33 ± 1.33) × 109/L vs.(8.38 ± 1.90) × 109/L,(25.52 ± 5.22) U vs.(31.83 ± 5.44) U and (632.80 ± 82.51) μg/g vs.(846.42 ± 11.10) μg/g,and there were statistical differences (P＜0.01 or ＜:0.05).Pearson correlation analysis result showed that,in active children,the UCEIS had high positive correlation with fecal calprotectin (r =0.707,P＜0.01),mild positive correlation with p-ANCA,ESR,IL-6,CRP and albumin (r =0.660,0.650,0.626,0.592 and 0.486;P＜0.01),and low positive correlation with PCT and white blood cell (r =0.362 and 0.245,P＜0.01 or ＜0.05).ROC curve analysis result showed that the optimal cut-off value of p-ANCA was 23.40 U,and the area under curve (AUC) in diagnosis of active stage UC was maximum (0.923),with a specificity of 93.1％ and a sensitivity of 78.9％;the optimal cut-off value of fecal calprotectin was 732.69 μg/g,and the AUC in diagnosis of active stage UC was maximum (0.937),with a specificity of 93.0％ and a sensitivity of 92.9％.Conclusions Serum p-ANCA is useful for UC disease activity diagnosis in children,while fecal calprotectin is independent predictor of the severe of UC.
ABSTRACT
Objective@#To investigate knowledge, attitudes, and practice on exercise and dietary behaviors among the primary and middle school pupils in Shandong province, and to provide a theoretical basis for a targeted education on diet and physical health among the primary and middle school students.@*Methods@#A questionnaire survey was conducted among 6 161 students from 3 cities in Shandong province by random cluster sampling.@*Results@#Self-reported rate of having breakfast every day for primary and middle school students were 85.0% and 75.3% ,respectively(χ2=84.0，P<0.01). 41.2% of primary school students were reported to do physical exercises more than 60 minutes every day and the rate was only 29.6% for middle school students(χ2=270.0，P<0.01). 39.2% of boys were reported to do physical exercises more than 60 minutes every day and the rate was 28.7% for the girls(χ2=90.4，P<0.01). The awareness rates of knowledge about dietary and physical exercise among primary and middle school students were 44.4% and 55.8%, respectively(χ2=702.0，P<0.01). The awareness rates among boys and girls were 50.4% and 51.9% respectively (χ2=12.4，P<0.01). Parents’ occupation, knowledge score and education level of mother were influencing factors of breakfast behavior everyday. The physical exercise was influenced by gender, parents' occupation, knowledge score and education level of mother.@*Conclusion@#It is necessary to educate students to take healthy diet and have more physia1 activities. Families, schools and government departments need to take feasible measures to ensure the time and intensity of pupils’ physical exercise through many ways.
ABSTRACT
Objective To investigate the changes of serum myocardial enzyme and immune function in chil-dren with bronchial asthma,and to evaluate the clinical value of level detection.Methods 100 children with bronchial asthma were divided into acute attack group and remission group,50 cases in each group,50 healthy children were selected as control group;the level of serum myocardial enzyme(AST,LDH,CK,CK-MB)and Tlymphocyte subsets(CD3+,CD4+,CD8+,CD4+/CD8+)were compared between the 3 groups,and to ana-lyze the correlation between levels and disease.Results The levels of AST,LDH,CK,CK-MB,CD4+and CD4+/CD8+in the children with asthma exacerbation and remission were significantly higher than those in the control group,and levels of acute attack group was significantly higher than the remission group,the difference was statistically significant(P<0.05).The level of CD8+in the acute attack group and remission group was significantly lower than that in the control group,and the level of the acute attack group was signif-icantly lower than that of the control group,the difference was statistically significant(P<0.05).Severity of bronchial asthma was significant positive correlation with AST,LDH,CK,CK-MB,CD4+and CD4+/CD8+(P<0.05),the severity of bronchial asthma was negatively correlated with the level of CD 8+(P<0.05).Conclu-sion There were different degrees of myocardial damage and abnormal immune function in children with bronchial asthma.With the aggravation of the disease,the level of myocardial enzyme and immune function of children with asthma were more significantly abnormal.
ABSTRACT
ObjectiveTo explore the chnical application value of ultrafine electronic endoscopy on the diagnosis of pediatric diseases of upper digestive tract.MethodsSeventy-five cases suspected upper gastrointestinal diseases from January 2009 to June 2010 were selected and were divided by systematic sampling method into observation group(47 cases) which was diagnosed with ultrafine electronic endoscopy and control group(28 cases) which was diagnosed by normal endoscopy.The data of diagnosis and compliance of two groups were observed and compared.ResultsCompliance rate of observation group and control group had significant difference[ 100.0％(47/47) vs.67.9％(19/28),P ＜ 0.01 ].The incidence rates of nausea,salivation and restless of observation group [ 8.5％(4/47 ),6.4％(3/47),8.5％(4/47 ) ]were lower than those of control group [39.3％(11/28),28.6％(8/28),50.0％(14/28)](P＜ 0.01 or ＜ 0.05).Correct diagnosis rate of control group was 84.2％ (16/19),while that of observation group was 95.7％ (45/47),but comparison of diagnosis showed no significant difference(P ＞ 0.05).The incidence rates of diaphragmatic spasm,sore throat and slow heartbeat in successful examination cases of control group[21.1％ (4/19),31.6％ (6/19),21.1％ (4/19) ]significant higher than those of observation group [0,2.1％ ( 1/47),2.1％(1/47) ](P ＜ 0.01 or ＜ 0.05 ).ConclusionUltrafine electronic endoscopy can achieve the same accuracy as normal endoscopy,but compared with normal endoscopy,it can improve the compliance of children during the examination and reduce the incidence of postoperative complications.