ABSTRACT
AIM: To estabish a RP-HPLC method for determing geniposidic acid、chlorogenic acid、geniposide in Duzhong Pingya Tablet(Folium Eucommiae).METHODS:Geniposidic acid,chlorogenic acid and geniposide were analysed by using C_(18) column with the mobile phase of methanol-water-aceacid(15∶85∶1.5) at the flow rate of 1 mL/min,the detective wavelength was set at 237 nm. RESULTS: The analytical had good linearity.The precision of geniposidic acid,chlorogenic acid and geniposide were 1.93%、1.50%、1.66%,respectively and the recoveries were 97.3%,97.2%,96.7%,respectively. CONCLUSION: This method is simple,quick and reproducible and could be used for the determination of three constituents in Duzhong Pingya Tablet.
ABSTRACT
AIM:To determine terpenelactonesines content in Yinxing Tongzhi Dripping Pills(ginkgoflavone,ginkgolide).METHODS:HPIC with evaporative light scattering detector(ELSD) was established to determine terpenelactones content in Yinxing Tongzhi Dripping Pills..The analysis was carried out on Angilent Hypersil ODS(5 ?m,4 mm?250 mm) column.The mobile phase consisted of THF-MeOH-H_2O(10:20:70).The operating conditions of the ELSD were a nebulizer nitrogen,flow-rate of 2.51 L/min and an evaporator tube temperature of 103(?C).RESULTS:No interference was found in the terpenelactones peak position.The method was simple,with a good linear relationship.The relative standard deviation of reproducibility about BB、GA、GB、GC was 1.33%,(1.79%,)1.92%,1.58% respectively and the average recoveries were 97.0%,96.5%,96.3%,97.6%,respectively.CONCLUSION:The method can be used for quality control.