Effect of laser therapy combined with biomaterials for treatment of periodontal bone defects

Objective: Evidence has shown that laser therapy can have a positive effect on the regeneration of periodontal tissues and improve the postoperative period through its antiinflammatory properties. The aim of the present review was to assess the effects of low-level and high-level laser in combination with different biomaterials on the regeneration of periodontal tissues in infrabony defects and furcation lesions. Materials and Methods: An electronic search of the PubMed database was performed, using ten specific descriptors. The search strategy gave 232 papers, 45 of which were initially selected based on the title, and following the reading of the abstracts, six of them were selected. After a complete reading of the texts, two papers were excluded for not fulfilling the inclusion criteria. Results: Thus, four papers were included in the present review, two of which employed low-level laser and two employed high-level laser in combination with enamel matrix derivative and bioactive glass. Non-significant results were found in the comparison of irradiated and non-irradiated groups using high-level laser, whereas statistically significant differences favoring the irradiated group were found with the use of low-level laser. Conclusions: Based on the data analyzed, low-level laser therapy has a positive effect on the regeneration of periodontal tissues, while high-level laser seems not to enhance the effect of enamel matrix derivative in the regeneration process.

Pro domain peptide of HGCP-Iv cysteine proteinase inhibits nematode cysteine proteinases

Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propeptide to inhibit proteinase in its active form. The hgcp-Iv cDNA sequence encodes a polypeptide of 374 amino acids with the same domain organization as other cathepsin L-like CPs, including a hydrophobic signal sequence and a pro domain region. HGCP-Iv, produced in Escherichia coli as a fusion protein with thioredoxin, degrades the synthetic peptide benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin and is inhibited by E-64, a substrate and inhibitor commonly used for functional characterization of CPs. Recombinant propeptides of HGCP-Iv, expressed in E. coli, presented high inhibitory activity in vitro towards its cognate enzyme and proteinase activity of Meloidogyne incognita females, suggesting its usefulness in inhibiting nematode CPs in biological systems. Cysteine proteinases from other species produced no noticeable activity.

Anestesia pelo haloéter com baixo fluxo de gases / Azeotropic mixture in low flow and closed system anesthesia

O presente trabalho avalia o tempo e o consumo do haloéter, em relaçäo ao halotano, usando baixo fluxo de oxigênio, durante as fases de induçäo, menutençäo e regressäo da anestesia e observa também a incidência de vômitos. Foram estudados 20 pacientes, adultos de ambos os sexos, estado físico ASA I e II, submetidos a vários tipos de cirurgias eletivas, divididos em dois grupos. A técnica anestésica constou de induçäo com tiopental, concentraçöes crescentes do agente volátil, ventilaçäo manual em sistema circular com absorvedor de dióxido de carbono e vaporizador de borbulha, reduzindo-se o fluxo para 0,3 a 0,91. min elevado a menos um de oxigênio a 100 por cento durante a manutençäo. Os resultados mostraram um consumo 25 a 29 por cento superior para o haloéter em relaçäo ao halotano, porém, com economia de 7 a 13 por cento do halotano contido no haloéter. Apenas dois pacientes, do grupo do halotano apresentaram náuseas e vômitos no pós-operatório. Em ambos os grupos, os parâmetros cardiovasculares se mantiveram estáveis, com discreta queda após a induçäo. O maior tempo de latência e regressäo do haloéter é compensado pelos efeitos simpaticomiméticos e analgesia do éter da mistura azeotrópica. Concluimos que o método proposto é prático, econômico, antipoluente e pode ser realizado em qualquer hospital, porque näo necessita de equipamentos sofisticados e caros

Hipotensäo arterial grave após medicaçäo pré-anestésica com Inoval / Severe hypotension following pre-medication with Inovar

The authors describe a case of several hypotension in a healthy, 19 years old man, scheduled for surgical correction of a short Achiles tendon, following premedication with 1 ml of Inovar i.m. The patient was premedicated 20 minuts before being admitted to the O. R. the patient was drowsy, the blood pressure was 50 x 25 mm Hg and the heart rate 60 bpm. The hypotension did not respond either to atropine (1 mg) or to fluid therapy with lactated Ringer. After 2 mg of paredrinol i.v., the patient developed junctional rhythn for to minutes followed by recovery of the sinus rhythm and of blood pressure to 110 x 80 mm Hg

A anestesia no Sarah- Instituto Nacional de Medicina do Aparelho Locomotor: visita pre-anestesica. / The anesthesia in Sarah-Instituto Nacional de Medicina do aparelho locomototor: pre-anesthesic visit

A participacao do anestesiologista no conhecimento da problematica do paciente cirurgico tem sido nossa preocupacao constante. Desde julho de 1978, criou-se a reavaliacao conjunta dos pacientes cirurgicos, na semana anterior a data prevista da cirurgia, da qual participam anestesiologistas, cirurgioes e colegas consultores Os pacientes sao entrevistados pelos anestesiologistas, seus prontuarios sao revistos, faz-se um exame clinico dirigido e discutem-se os problemas pertinentes com os cirurgioes ou consultores e os pacientes recebem a orientacao final. De 866 pacientes que foram vistos, 49 pacientes foram medicados, para reavaliacao na internacao. 36 cirurgias foram suspensas pelos anestesiologistas, por apresentarem diversas patologias. Dois pacientes foram internados para tratamento clinico hospitalar. Esta experiencia trouxe diversas contribuicoes: uma melhor avaliacao dos pacientes, evitou-se a marcacao de cirurgia sem adequado preparo; racionalizando-se o uso do centro cirurgico, melhora-se a possibilidade da aceitacao das tecnicas regionais em pacientes pediatricos e melhora-se tambem o relacionamento cirurgiao -anestesiologista, contribuindo para a unidade de pensamento e de conduta dos proprios anestesiologistas