Experiencia cubana en planificación de los recursos humanos de enfermería en la epidemia por COVID-19 / Cuban experience in human resources planning for epidemic nursing COVID-19

Introducción: la pandemia por el virus SARS-CoV-2 ha provocado numerosas víctimas en el plano mundial. A partir de los primeros casos diagnosticados en Cuba, la máxima Dirección del Estado y el Ministerio de Salud Pública dispusieron el reordenamiento de los servicios del Sistema Nacional de Salud para garantizar la atención a los pacientes confirmados con la enfermedad y los programas priorizados con el aseguramiento de los recursos humanos de enfermería, además del desarrollo de acciones para garantizar la continuidad y calidad de las prestaciones proporcionadas. Desarrollo: se organizaron grupos de pesquisa diaria del 100% de la población en las viviendas para identificar a pacientes sintomáticos y se crearon centros para la vigilancia de acuerdo con la clasificación del paciente, ya fuera con- tacto, sospechoso o confirmado. En estos centros, el personal de enfermería mantiene una vigilancia para la detección oportuna de síntomas o agravamiento del estado de salud y efectúa el control de las pruebas diagnósticas por PCR evolutiva y el cumplimiento de los tratamientos médicos hasta su alta. En los hospitales se atienden los casos confirmados y sospechosos de alto riesgo de COVID-19. En estas instituciones se estableció un sistema de trabajo-vigilancia-descanso con el objetivo de garantizar la atención con el personal estrictamente necesario. Conclusiones: este trabajo permite compartir las experiencias vivenciales en la planificación del recurso humano de enfermería en cada una de las etapas y destaca la colaboración médica como muestra de solidaridad y humanismo que caracteriza a la sociedad. Los resultados obtenidos permitirán establecer líneas de trabajo con una clara visión de este fenómeno, con utilización óptima de los recursos materiales y humanos, además de contribuir a la preparación de los profesionales para el intercambio y la experiencia con otros países.

Introduction: The pandemic caused by the SARS-CoV-2 virus has hit numbers of victims worldwide, from the first cases diagnosed in Cuba, the highest state administration and the Ministry of Public Health provides the reorganization of the services of the National System of Health, guaranteeing care for patients confirmed with the disease and prioritized programs with the assurance of nursing human resources and the development of actions to guarantee the continuity and quality of the services provided for what the purpose is outlined. Development: Daily investigation groups of 100% of the population were organized in the homes to detect symptomatic patients, surveillance centers were created according to the classification of the patient as contact, suspect and confirmed, in these centers the nurse maintains a surveillance for the timely detection of symptoms or worsening of their health, controls the performance of the evolutionary PCR and compliance with medical treatments until discharge. In hospitals, confirmed and suspected high-risk cases of COVID-19 are treated. In these institutions, a work-surveillance-rest system was established in order to guarantee care with the strictly necessary personnel. Conclusions: In this article we share the experiential experiences in the planning of the nursing human resource in each of the stages and medical collaboration stands out as a sign of solidarity and humanism that characterizes our society. The results obtained will allow establishing lines of work with a clear vision of this phenomenon with optimal use of material and human resources, as well as contributing to the preparation of professionals with a view to exchange and experience with other countries.

Identification of miRNAs linked to peanut nodule functional processes

microRNAs (miRNAs) are non-coding small RNAs that regulate gene expression at post-transcriptional level.Thousands of miRNAs have been identified in legumes, but studies about miRNAs linked to peanut nodulefunctionality are scarce. In this work we analyzed transcriptional changes in peanut nodules to identifymiRNAs involved in functional processes of these organs. We found 32 miRNAs precursors differentiallyexpressed in nodules compared with roots, and predicted the potential targets of their corresponding maturemiRNAs. Among them, 20 belong to 14 conserved miRNAs families and 12 are Arachis hypogaea-specificmiRNAs. Expression levels of 3 miRNAs (ahy-miR399, ahy-miR159 and ahy-miR3508) were confirmedexperimentally by qPCR. We also demonstrated that the expression of these miRNAs was not affected byinoculation of a biocontrol bacterium or a fungal pathogen. The catalogue of differentially expressed miRNAprecursors and the expression of the corresponding mature miRNA potential targets in the nodules of A.hypogaea obtained in this work is a database of strong candidates, including A. hypogaea-specific miRNAs, forthe regulation of the nodule functionality. The analysis of their role in this process will certainly lead to thecharacterization of essential regulators in these particular aeschynomenoid nodules.

Capacidades para la gestión de resultados de investigación en sistemas y servicios de salud de enfermería en Cuba / Capacities for managing the results of health systems and health services research by nurses in Cuba

Introducción: Los resultados de las investigaciones en sistemas y servicios de salud trascienden el alcance de los objetivos previstos. Su gestión permite el cierre del ciclo investigativo y es para los profesionales de enfermería en Cuba uno de los retos a vencer en la visualización de su práctica investigativa. Por ello, se desarrolló una experiencia de la práctica que contribuyó a la consolidación de la gestión de los resultados de las investigaciones en sistemas y servicios de salud por los profesionales de enfermería en Cuba. Desarrollo: Se llevaron a cabo talleres con el objetivo de desarrollar en los profesionales de enfermería cubanos las capacidades institucionales e individuales para la gestión de los resultados de las investigaciones en sistemas y servicios de salud. Las primeras se hicieron con la designación de un responsable de investigación para la gestión institucional de este tipo de estudio y la creación de un repositorio que compilara los resultados de las investigaciones existentes. Las segundas recayeron en los responsables seleccionados y estuvieron relacionadas con la obtención de la categoría de investigadores, la gestión de publicación de artículos, la protección legal de resultados y su registro oficial en el sistema nacional de investigaciones del país. Conclusiones: las capacidades desarrolladas contribuyeron a la gestión de los resultados de los profesionales de enfermería de las instituciones participantes. Estas se integran a la etapa de sostenibilidad que implica el nivel nacional de capacidades para la investigación.

Introduction: The results of research in health systems and health services go beyond the significance of the expected target. Managing these results allows the closure of the research cycle and is one of the challenges that Cuban professional nurses have to confront in order to make visible their results. Due to this situation, a practical experience was developed to consolidate the management of research results in health systems and health services. Development: Workshops were carried out in order to develop institutional and individual capacities in Cuban professional nurses so that they could manage the results of health Systems and health services research. Institutional capacities were developed by designating a professional nurse researcher, responsible for the institutional management of this kind of studies and the creation of a repository to gather the results of the existent research. Individual capacities were carried out by the selected professionals and had to do with obtaining the researcher category, the publication of results, the legal protection of results, and the official registration in the national research system. Conclusions: The developed capacities contributed with the management of the research results of professional nurses of each participating institution. Those capacities are part of the sustainability stage that includes the national level of capacities for research.

Interrelationships between Bacillus sp. CHEP5 and Bradyrhizobium sp. SEMIA6144 in the induced systemic resistance against Sclerotium rolfsii and symbiosis on peanut plants.

Plant-growth-promoting bacteria are often used to enhance crop yield and for biological control of phytopathogens. Bacillus sp. CHEP5 is a biocontrol agent that induces systemic resistance (ISR) in Arachis hypogaea L. (peanut) against Sclerotium rolfsii, the causal agent of root and stem wilt. In this work, the effect of the co-inoculation of Bacillus sp. CHEP5 and the peanut nodulating strain Bradyrhizobium sp. SEMIA 6144 was studied on induction of both systemic resistance and nodulation processes. Bradyrhizobium sp. SEMIA 6144 did not affect the ability of Bacillus sp. CHEP5 to protect peanut plants from S. rolfsii by ISR and the priming in challenged-plants, as evidenced by an increment in phenylalanine ammonia-lyase enzyme activity. Additionally, the capacity of Bradyrhizobium sp. SEMIA 6144 to induce nodule formation in pathogen-challenged plants was improved by the presence of Bacillus sp. CHEP5.

Correlation between Plasmodium vivax variants in Belém, Pará State, Brazil and symptoms and clearance of parasitaemia

The aim of this study was to determine how different types of P. vivax affect clinical symptoms and parasitaemia clearance. Blood was collected from individuals from Pará State, Brazil. The patients were treated as chloroquine plus primaquine. P. vivax were typed daily till D7 and again on D30. Now we can confirm a previously reported correlation between P. vivax genotype and response to chloroquine. Clinical symptoms do not allow for objective identification of different P. vivax types in the Brazilian Amazon, since the VK247 and P. vivax-like have only been detected in mixed infections

Performance of an immunochromatography test for virax malaria in the Amazon region, Brazil

Avaliaçäo do teste ICT malaria Pf/PvTM para o diagnóstico da malária por P. vivax em Belém, Estado do Pará. Foram comparados os resultados do teste imunocromatográfico com a gota espessa (GE) e avaliados o comportamento desse teste, estocado a três temperaturas distintas (250C/ 300C/ 370C), 24 horas antes de seu uso. A sensibilidade do ICT malaria Pf/PvTM foi de 61,8 por cento com especificidade de 100por cento, valores preditivo positivo e negativo de 100 por cento e 71,8 por cento, respectivamente, e acurácia de 80,67por cento. A sensibilidade desse teste foi independente da densidade parasitária. Este teste necessita de reavaliaçäo para melhorar o seu comportamento no diagnóstico da malária por P. vivax

Associação entre líquen plano oral e infecção pelo vírus da hepatite C: revisão de literatura / Association between oral lichen planus and hepatitis C virus infection: a literature review

Estudos epidemiológicos têm demonstrado que existe associação entre o líquen plano oral (LPO) e a infecção crônica pelo vírus da hepatite C (HCV). O RNA do HCV tem sido detectado nas células epiteliais das lesões de LPO em pacientes infectados, através da técnica da reação em cadeia da polimerase por transcrição reversa (RT-PCR) e por hibridização in situ. Os autores fazem uma revisão da literatura sobre as evidências da implicação do HCV na etiologia do LPO, discutindo alguns aspectos controversos sobre o tema

A participacao popular na gerencia do Sistema Unico de Saude: erros e acertos da experiencia de Botucatu - SP / Popular participation in management of the Unified Health System: wrong and right points of Botucatu - SP, Brazil

Relata a experiencia de participacao de representantes da comunidade de Botucatu na gerencia do Sistema Unico de Saude (MMSC)