Efecto del extracto vegetal JV-001 sobre elementos sanguíneos concapacidad fagocítica / Effect of the plant extract JV-001 on blood elements with phagocytic capacity

INTRODUCCIÓN: La respuesta inmune combina una serie de señales y mecanismos complejos para producirse, las que pueden ser moduladas y potenciadas. Esto, según el conocimiento popular es posible empleando extractos vegetales, siendo uno el extracto vegetal JV-001. Éste demostró, en estudios previos, la capacidad de activar y mejorar la respuesta inmune celular de origen murino, pero su efecto a nivel leucocitario humano es desconocido; debido a esto, el objetivo de la investigación fue estudiar el efecto del JV-001 en leucocitos humanos. MATERIAL Y MÉTODO: Para este trabajo se extrajo sangre heparinizada a varones sanos, ésta fue tratada, en primera instancia, con NH-4Cl 64 por ciento para obtener leucocitos totales, y en otra, separada su fracción mononuclear mediante gradiente de Histopaque®. Las células obtenidas se ajustaron a 8x106; alicuotas de 100 uL de JV-001 o PBS. La placa se incubó a 37ºC por 30 minutos, luego se lavaron y la peroxidasa remanente en cada pocillo se determinó revelándola con Ortofenilendiamina y H2O2, expresando lo obtenido como absorbancia leída a 450 nm. RESULTADOS: Se identificó un aumento en la adhesión de leucocitos totales entre un 44,35 por ciento y 63,3 por ciento expresado mediante la obtención de un delta positivo de absorbancia con respecto a los controles. DISCUSIÓN: Se desconoce el mecanismo que incrementa la adhesión, pero probablemente obedece al aumento en la expresión de integrinas. Es relevante destacar que este aumento no ocurre al incubar las células mononucleares o polimorfonucleares por separado, sugiriendo que el principio activo de JV-001 no actúa directa ni únicamente sobre células adherentes.

INTRODUCTION: The immune response combines a series of signals and complex mechanisms to occur, which can be modulated and enhanced. This one according the popular knowledge is possible using plant extracts, being one of them the plant extract JV-001. This showed, in previous studies, the ability to activate and enhance the cellular immune response of murine origin, but its effect on human leukocyte level is unknown, so the objective of this work was to study the effect of JV-001 in human leukocytes. MATERIAL AND METHOD: In this paper was used heparinized blood of healthy males, at first instance, this was treated with NH4Cl 64 percent to obtain total leukocytes, and in another, separate the mononuclear fraction by Histopaque®gradient. The cells obtained were adjusted to 8x106; aliquots of 100 uL of JV-001 or PBS. The plate was incubated at 37ºC for 30 minutes and then washed, the peroxidase remaining in each recipient was revealed and determined with Orthophenylenediamine and H2O2, expressing the proceeds as absorbance read at 450 nm. RESULTS: The results showed an increase in total leukocyte adhesion between 44.35 percent and 63.3 percent expressed by obtaining a positive delta absorbance with respect to controls. DISCUSSION: The mechanism that increases the adhesion is unknown, but probably due to increased expression of integrins. It is important to note that this increase does not occur upon incubation of mononuclear or polymorphonuclear cells separately, suggesting that the active principle of JV-001 not only does not act directly on adherent cells.

Intervención del flagelo de campylobacter jejuni subsp. jejuni en la adherencia a cultivos celulares: evidencias bacteriológicas e inmunológicas / Intervention of campylobacter campylobacter jejuni subsp. jejuni flagella in the adhesion to cellular cultures: bacteriological and immunological evidences

The participation of the flagella of a virulent strain (O52) of campylobacter jejuni subsp. jejuni in the adhesion to HEp-2 cells and their inhibition by means of homologous polyclonal antibodies, moniclonal antiflagella antibodies and colostral natural antibodies (IgA) was studied. An aflagellated strain (T1) was used as negative control. Adhesion was observed in higher rates with O52 strain (72 percent) than with T1 strain (27,5 percent). Polyclonal, monoclonal and colostral antibodies inhibited O52 strain adhesion in more than 70 percent (p<0,001). T1 strain adhesion was inhibited only by polyclonal and colostral natural antibodies. Our results suggest that the flagella of C. jejuni subsp. jejuni could participate effectively in the adhesion process. However, the inhibition of T1 strain by polyclonal and colostral antibodies suggest the existence of other kinds of adhesins in the bacterial surface