Potential Role Of Annona Squamosa Linn Extract And N-Acetyl Cysteine In Management Of Rifampicin-Induced Hepatoxicity In Rats

Objective: Rifampicin (RIF) could be a recognized therapeutic and preventive agent against tuberculosis. Still, high rates of many side effects and symptoms related to hepatotoxicity have identified during treatment. So, the current study was aimed to evaluate the antioxidant and hepatoprotective activity of methanolic extract of Annona Squamosa Linn (MEAS) and N-Acetyl Cysteine (NAC) against RIF induced hepatic injury in male rats. Methods: The hepatoprotective effects of MEAS (500 mg/kg b.wt.) and NAC (100 mg/kg b.wt.) or co-treatment were assessed in a model of hepatotoxicity by RIF (300 mg/kg b. wt.) in male rats daily for 21 d. Moreover, bilirubin, total protein, albumin, ALT, AST, ALP, GGT, MDA, and GSH were estimated. In addition, the levels of IL-6, IL-10, 8(OH)dG, and Bcl2 were evaluated. Results: The oral administration of MEAS and NAC or their combination resulted in significant reductions in the levels of bilirubin, albumin, hepato-specific markers namely ALT, AST, ALP, GGT, and MDA as compared to the RIF group. Furthermore, MEAS and NAC or the combination of MEAS and NAC treatment significantly up-regulated the levels of total protein, glutathione reductase with concomitant decrease in inflammatory marker level IL-6 and apoptotic marker level 8(OH)dG as well as increment the level of anti-inflammatory marker IL-10 and anti-apoptotic marker Bcl2 as compared to the RIF group. Histological examination of the liver tissue indicated that co-treatment with MEAS and NAC completely abolished the inflammation and degeneration in hepatocytes and restore the liver tissue to its normal structure. Conclusion: The present findings demonstrated that a co-treatment of MEAS and NAC seems to be more productive and curative than alone MEAS or NAC treatment and strongly compensated the liver damage induced by RIF.

Hyperthyroide chez les malades traites par Amiodarone a Yaounde: a propos de 6 observations.[Lettre]

6 dossiers de malades sous Amiodarone ayant une hyper-thyroide par surcharge iodee sont analyses; ils representent 10 pour cent de la population d'hyperthyroidies en 4 ans. L'Amiodarone a ete prescrite comme anti-arythmique et anti-angoroux; apres une longue amelioration; l'aggravation de l'arythmie et l'angor a permis de diagnostiquer l'hyperthyroidie par surcharge iodee. En l'absence d'investigation scintigraphique en Afrique; l'iodurie de 24 heures elevee; les hormonemies thyroidiennes elevees; la TSH basse et l'absence d'anticorps antithyroidiens permettent de poser le diagnostic. L'evolution est en general bonne; apres sevrage de l'Amiodarone et mise sous propylthiouracile et corticoide. Les auteurs concluent que le malade sous Amiodarone en Afrique noire; comme ailleurs; doit beneficier d'une surveillance attentive; notamment pour la complication majeure qu'est l'hyperthyroidie par surcharge iodee