ABSTRACT
The human orosomucoid 1 gene (ORM1) codes an alpha-1-acid glycoprotein that has been classified as an acute-phase reactive protein, and a major drug-binding serum component, as well as an immunomodulatory protein with genetic polymorphisms. Evaluation of ORM variation through isoelectric focusing and immunobloting has revealed a world-wide distribution of the ORM1 F and ORM1 S alleles. We evaluated and examined the genetic characteristicsof two Mexican populations that have different anthropological and cultural antecedents, examining two ORM1 genotypes (exon 1 - A/G (Gln20Arg) and exon 5 G/A (Val156Met)) in 145 individuals, using nested polymerase chain reaction, sequencing, and restrited fragment length polymorphism. Mexican Mestizos had higher frequencies of the exon 1 A allele (P = 0.020) and AA genotype(P = 0.018) and lower frequency of the G allele (P = 0.020) when compared to Teenek Amerindians. When we examined exon 5 G/A (Val156Met) polymorphisms, we found significantly higher frequencies of the G allele (P = 0.0007) and the GG genotype (P = 0.0003) in the Mexican Mestizo population. The Teenek population had a significantly higher frequency of the A allele than has been reported for Chinese and African (P < 0.05) populations, and the G/A genotype was more frequently found in this Mexican population than in Chinese, African and European populations (P < 0.05).
Subject(s)
Humans , Exons/genetics , Genetics, Population , Indians, North American/genetics , Orosomucoid/genetics , Polymorphism, Genetic , Alleles , DNA , Gene Frequency , Genetic Variation , Mexico , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Sequence Analysis, DNA , Statistics as TopicABSTRACT
Diez pacientes con estenosis mitral fueron estudiados mediante cateterismo cardiaco derecho e izquierdo. A fin de determinar las propiedades diastolicas del ventriculo izquierdo se utilizo una tecnica hemodinamica-ecocardiografica, con la que se registraron simultaneamente la presion intraventricular y un ecocardiograma a nivel del cuerpo del ventriculo izquierdo. El volumen ventricular fue medido determinando el diametro menor directamente del eco ventricular y asumiendo el diametro mayor (L) de la relacion L/T obtenida de la cineventriculografia izquierda. Con una microcomputadora se obtuvo una curva suavizada de volumen-tiempo, su derivada y un asa de presion-volumen. La porcion diastolica exponencial del asa fue utilizada para obtener un indice de rigidez ventricular a volumen cero. El volumen diastolico y la fraccion de expulsion fueron normales. El llenado ventricular se encontro disminuido y la rigidez ventricular normal. Se concluye que el metodo propuesto permite facilmente la obtencion de datos simultaneos de presion-volumen, que el procesamiento de datos complejos puede hacerse con una microcomputadora de bolsillo y que no hay un defecto de las propiedades elasticas del ventriculo