ABSTRACT
The composition of six samples of essential oil (EO) extracted from leaves, flowers and seeds of several plants of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, was investigated by GC/MS and GC/FID. 1,8-Cineole, spathulenol, α-pinene, β-pinene were the major constituents. Ten constituents that have not been previously described in the composition of the oil of H. fruticosa were identified. Hydrocarbons sesquiterpenes represented the main group, followed by hydrocarbons monoterpenes. The results were submitted to Cluster Analysis which allowed three groups of EO to be distinguished with respect to the content of α-pinene/β-pinene, 1,8-cineole and spathulenol. Growth stages of the plants and geographical parameters seem to be important factors determining the variability of the oil. Sesquiterpenes were mainly produced in the seeds.
ABSTRACT
Various species of Hyptis are used in folk medicine as anti-inflammatory. In order to evaluate the actions of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, studies were performed on anti-inflammatory and antioxidant activities. The ethanol extract (EE) of H. fruticosa leaves and its n-CH, CHCl, EtOAc, and MeOH/HO partitions were used in the following experiments. Oral treatment with the EE of H. fruticosa leaves (100, 200, and 400 mg/kg) or its n-C6H14, EtOAc, and MeOH/H2O partitions (50, 100, and 200 mg/kg) elicited inhibitory activity (p<0.05) on carrageenan-induced oedema formation and leukocyte migration into the peritoneal cavity in rats. However, the CHCl3 partition did not show any inhibitory effect on paw oedema and peritonitis experimental models. The EE and EtOAc partition present highest antioxidant potential (IC50 = 35.00±1.01 and 36.67±2.65 µg/mL DPPH, respectively), similar to the reference compound (IC50 = 16.67±1.21 µg/mL). In conclusion, H. fruticosa shows anti-inflammatory and antioxidant activities.
Várias espécies do gênero Hyptis são utilizadas na medicina popular para tratar processos inflamatórios. Para avaliar as ações anti-inflamatória e antioxidante da Hyptis fruticosa Salzm. ex Benth., Lamiaceae, utilizou-se extrato etanólico (EE) das folhas desta planta e suas partições n-C6H14, CHCl3, AcOEt e MeOH/H2O. O tratamento oral com o EE das folhas da H. fruticosa (100, 200 e 400 mg/kg) ou suas partições n-C6H14, AcOEt e MeOH/H2O (50, 100 e 200 mg/kg) apresentou atividade inibitória sobre a formação de edema e migração leucocitária para a cavidade peritoneal induzidas pela carragenina em ratos (p<0,05). Entretanto, a partição CHCl3 não causou nenhum efeito sobre a formação de edema e migração de células peritoneais. O EE bruto e a partição AcOEt apresentaram alto potencial antioxidante (IC50 = 35,00±1,01 e 36,67±2,65 µg/mL DPPH, respectivamente), similar ao composto referência (IC50 = 16,67±1,21 µg/mL). Em conclusão, demonstrou-se que a H. fruticosa apresenta atividades anti-inflamatória e antioxidante.