ABSTRACT
Proteomics correspond to the identification and quantitative analysis of proteins expressed in different conditions or life stages of a cell or organism. Methods used in proteomics analysis include mainly chromatography, two-dimensional electrophoresis and mass spectrometry. Data generated in proteomics analysis vary significantly, and to identify a protein it is often necessary to perform a series of experiments, comparing its results to those found in proteomics databases. Existing proteomics databases are usually related to only one type of experiment or represent processed results, not raw data. Therefore, proteomics researchers frequently have to resort to several data repositories in order to be able to perform the identification. In this paper, we propose an integrated proteomics and transcriptomics database that stores raw and processed data, which are indexed allowing them to be retrieved together or individually. The proposed database, dubbed BNDb for Biomolecules Nucleus Database, is implemented using an MySQL server and is being used to store data from the parasite Schistosoma mansoni, the scorpion Tittyus serrulatus and the spider Phoneutria nigriventer. The database construction uses a relational approach and data indexes. The data model proposed uses groups of tables for each data subtype, which store details regarding the experimental procedure as well as raw data, analysis results and associated publications. BNDb also stores transcriptomics data publicly available which are associated with identifications performed on new samples. By using BNDb, we expect not only to contribute to proteomics research but also to provide a useful service for the scientific community.
Subject(s)
Animals , Databases, Nucleic Acid , Databases, Protein , Proteomics/methods , Transcription, Genetic , Database Management Systems , User-Computer InterfaceABSTRACT
Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used routinely for many years for DNA sequencing, it has not been used in the in vitro primer extension assay. The use of fluorescence labels has obvious advantages over radioactivity, including safety, speed and ease of manipulation. In the present study, we demonstrated the potential of non-radioactive in vitro primer extension for DNA polymerase studies. By using an M13 tag in the substrate, we can use the same fluorescent M13 primer to study different substrate sequences. This technique allows quantification of the DNA polymerase activity of the Klenow fragment using different templates and under different conditions with similar sensitivity to the radioactive assay.
Subject(s)
Sequence Analysis, DNA , DNA Polymerase I/metabolism , Escherichia coli/enzymology , Fluorescein/metabolism , DNA Primers/metabolism , Automation , Hydrogen-Ion ConcentrationABSTRACT
Biomphalaria tenagophila is very important for schistosomiasis transmission in Brazil. However its mechanisms of interaction with Schistosoma mansoni are still scantly studied. Since this snail displays strains highly susceptible or completely resistant to the parasite infection, the knowledge of that would be a useful tool to understand the mechanism of snail resistance. Particularly, the Taim strain consistently shows absolute resistance against the trematode, and this resistance is a dominant character. A multidisciplinary research group was created aiming at studying B. tenagophila/S. mansoni interaction. The possibility for applying the knowledge acquired to obtain a biological model for the control of S. mansoni transmission in endemic areas is discussed.
Subject(s)
Humans , Animals , Biomphalaria , Disease Vectors , Host-Parasite Interactions , Schistosoma mansoni , Brazil , Schistosomiasis mansoniABSTRACT
Se evaluaron varias cepas de levaduras para determinar los rendimientos en ß galactosidasa. La mejor cepa productora resultó Criptococcus. La actividad enzimática de algunas cepas de Cr. albidus y Cr. luteolus fueron superiores a las cepas de levadura Kluyveromyces fragilis y K. lactis, que han sido reportadas en la literatura como las mejores fuentes de la enzima para su utilización en la industria alimenticia
Subject(s)
beta-Galactosidase , Yeasts , CubaABSTRACT
Para facilitar e melhorar o manejo de suínos, a induçäo e a sincronizaçäo de partos em porcas com uso de prostaglandina F2 alfa vêm dando resultado em muitos países. O presente trabalho, realizado no Peru, demonstra a adequaçäo do método para programar o nascimento de leitöes, obtendo leitegadas uniformes, que facilitam a boa administraçäo da granja porcina