ABSTRACT
A soil screened Bacillus flexus XJU-1 was induced to simultaneously produce alkaline amylase, alkaline lipase and alkaline protease at their optimum levels on a common medium under submerged fermentation. The basal cultivation medium consisted of 0.5% casein, 0.5% starch and 0.5% cottonseedoil as an inducer forprotease, amylase, and lipase, respectively. The casein also served as nitrogen source for all 3 enzymes. The starch was also found to act as carbon source additive for both lipase and protease. Maximum enzyme production occurred on fermentation medium with 1.5% casein, 1.5% soluble starch, 2% cottonseed oil, 2% inoculum size, initial pH of 11.0, incubation temperature of 37 °C and 1% soybean meal as a nitrogen source supplement. The analysis of time course study showed that 24 h was optimum incubation time for amylase whereas 48 h was the best time for both lipase and protease. After optimization, a 3.36-, 18.64-, and 27.33-fold increase in protease, amylase and lipase, respectively was recorded. The lipase was produced in higher amounts (37.72 U/mL) than amylase and protease about 1.27 and 5.85 times, respectively. As the 3 enzymes are used in detergent formulations, the bacterium can be commercially exploited to secrete the alkaline enzymes for use in detergent industry. This is the first report for concomitant production of 3 alkaline enzymes by a bacterium.
Subject(s)
Amylases/metabolism , Bacillus/enzymology , Bacillus/metabolism , Bacterial Proteins/metabolism , Detergents/metabolism , Endopeptidases/metabolism , Enzyme Inhibitors/metabolism , Lipase/metabolism , Bacillus/growth & development , Bacillus/isolation & purification , Carbon/metabolism , Culture Media/chemistry , Fermentation , Hydrogen-Ion Concentration , Nitrogen/metabolism , Soil Microbiology , Temperature , Time FactorsABSTRACT
Se presenta un caso de hemobilia postraumática con fistula arteriobiliar; se comenta la sintomatologia del cuadro clinico; se mencionan los medios diagnosticos para esta patologia rara; se preconiza el tratamiento por embolizacion selectiva y se hace una revision de la literatura sobre el tema, en la que se informa sobre 73 casos publicados hasta 1981 (4).