ABSTRACT
Aim To demonstrate that bigelovii E,one of the triterpenoid compounds isolated from Salicornia bigelovii Torr. ,induced apoptosis and inhibited prolif-eration of human breast cancer cells MCF-7. Methods MTT and Clonogenic were used to detect the anti-proliferative effect of bigelovii E on human tumor cells. Hoechst 33258 staining was used to observe the chan-ges of cell morphology in the treatment with bigelovii E. The effect of bigelovii E on cell apoptosis,cell mi-tochondrial membrane potential and the ROS level was analyzed by flow cytometry. The expression of mito-chondrial apoptotic pathway and its regulated proteins were analyzed by Western blot. Results Bigelovii E was most sensitive to MCF-7;bigelovii E had lower toxicity to normal cells HLF1;bigelovii E inhibited the colony formation of breast cancer MCF-7;the apoptosis pattern induced by bigelovii E was detected by Hoechst 33258;Western blot showed that bigelovii E could down-regulate the phosphorylation of mTOR,p70S6K and 4-EBP,up-regulate the expression of Bcl-xl and Mcl-1 proteins,promote the mitochondrial apoptosis pathway,reduce the mitochondrial membrane poten-tial,and induce ROS,leading to mitochondrial func-tion damage,and ultimately inducing apoptosis. Con-clusion Bigelovii E regulates mitochondrial apoptosis pathway through mTOR pathway,induces apoptosis and has a good anti-tumor effect.
ABSTRACT
Gastrointestinal bleeding is a common complication of gastrointestinal diseases and gastrointestinal surgery, which may lead to hemorrhagic shock or cause death if not treated properly and promptly.Currently, endoscopic treatment for gastrointestinal bleeding includes clip closure, endoscopical injection of saline/adrenaline, argon plasma coagulation (APC), electrocoagulation and heater probe coagulation etc..Endoscopic clip closure mainly includes traditional closure through-the-scope clip (TTSC) and more novel closure over-the-scope clip (OTSC).This article reviewed the use of OTSC in the treatment of gastrointestinal bleeding.
ABSTRACT
Post traumatic epilepsy （PTE） refers to the epileptic seizures after traumatic brain injury. Organic damage can be found by imaging examination, and abnormal electroencephalogram can be detected via electroencephalogram examination which has the similar location of the brain injury. PTE has the characteristics of low incidence, absence of case reports, and easy to exaggerate the state of illness, which add difficulties to the forensic identification. This paper reviews the status of epidemiology, pathogenesis, clinical treatment and forensic identification for PTE.
Subject(s)
Humans , Brain Injuries, Traumatic/physiopathology , Electroencephalography , Epilepsy , Epilepsy, Post-Traumatic/pathology , Forensic Pathology , IncidenceABSTRACT
OBJECTIVE@#To study the correlation of daily living activities with location and severity of trau- matic brain injury (TBI) and to provide a theoretical basis for improving the accuracy of expert opinion.@*METHODS@#Five hundred and one cases of patients with TBI were selected. Detailed records included following: pre-injury situation, location and severity of injury, treatment and education. Daily living activi- ties scale (Barthel index) was applied to test the subjects' daily living activities. The relevance among location and severity of TBI and Barthel index was statistically analyzed.@*RESULTS@#In mild TBI group, there was no significant difference in Barthel index among each location (P>0.05). In moderate TBI group, there were significant differences in Barthel index between subarachnoid hemorrhage and cerebral lobe injury, also between parietal, occipital lobes injury and frontal lobe injury, parietal, occipital lobes injury and temporal lobe (P<0.05), respectively, whereas no significant difference in Barthel index between frontal lobe injury and temporal lobe injury (P>0.05). In severe TBI, there were significant differences in Barthel index between every two different locations (P<0.05).@*CONCLUSION@#There is some correlation between the location of TBI and Barthel index, which provides an important reference value for analyzing and determining daily living activities after TBI.
Subject(s)
Adult , Female , Humans , Male , Activities of Daily Living , Brain Injuries/rehabilitation , Outcome Assessment, Health Care , Trauma Severity IndicesABSTRACT
Objective To compare the predicting values for Prognosis among Global Risk Classification (GRS),Synergy Between Percutaneous Coronary Intervention With TAXUS and Cardiac Surgery (SYNTAX) score,the European System for Cardiac Operative Risk Evaluation (EuroSCORE) in patients who received stenting because of unprotected left main coronary artery (ULMCA) lesion.Methods Totally 105 successive elderly patients with ULMCA lesion who received stenting were divided into 2 groups:with and without main adverse cardiac events (MACE).The clinical and angiographic characteristics were analyzed and then compared among GRC,SYNTAX score and EuroSCORE.Results As compared with none MACE group,MACE group had higher EuroSCORE score (2.0±2.3 vs.6.5±2.9,t=8.18,P=0.002),and more trivessel disease and left main bifurcation lesion (x2 =8.96,6.96,P =0.011,P =0.008).High risk GRC showed more MACE than medium or low risk GRC [55.9％ (19/34) vs.20.5％(9/44),7.4％ (2/27),x2 =19.77,P=0.001].AUC(95％CI )of GRC,SYNTAX score and EuroSCORE were [0.821 (0.730-0.912),0.586(0.462-0.709) and 0.631 (0506-0.757)],respectively.Compared with SYNTAX score and EuroSCORE,GRC was superior in the MACE predicting value (Z=3.29,2.63,P＜0.01 or P＜0.05).